Vector Database(addgene) 地址:http://www.addgene.org/ 这个网站很页面很人性化,以前叫做 lablife,现在网站做了整合,传送门 http://www.91bio.com/carriers-website-to-find/,比如查找 pRS 类质粒图谱,直接在搜索框输入 pRS,可以看到此类质粒一共有三十多个。 找到自己需要的质粒名称,点击进入,就可以看到质...
Here we describe “Supernova” series of vector systems that enable single-cell labeling and labeled cell-specific gene manipulation, when introduced by in utero electroporation (IUE) or adeno-associated virus (AAV)-mediated gene delivery. In Supernova, sparse labeling relies on low TRE leakage. ...
The tumor microenvironment (TME) is a complex amalgam of tumor cells, immune cells, endothelial cells and fibroblastic stromal cells (FSC). Cancer-associated fibroblasts are generally seen as tumor-promoting entity. However, it is conceivable that particular FSC populations within the TME contribute ...
over all chromosomes without hotspots (Fig.4dand Supplementary Data2), in line with the random AAV vector integration profiles in mice, NHPs and humans49,50,51, and arguing against clonal selection of AAV IS in vitro or in vivo. Of the 180 IS, 85 (47.23%) were within genes (Fig.4e,...
Starting from database searches of Ae. albopictus and the C6/36 cell line whole genome shotgun sequences for the prohibitin 2 (PHB2) gene, primers were designed to confirm the gene sequence in our laboratory-passaged C6/36 cell line for the correct design and cloning of CRISPR RNA into ...
There are some differences in gene names between the species to allow for comparison of the murine and human transcriptomic response. We used the BiomaRt getLDS function, which uses the Ensembl database to pair orthologous genes in mice and humans. To investigate the differences between murine and...
Availability and requirements Nucleotide sequence accession number and plasmid database information The sequence of pCM433 has been deposited [GenBank:EU118176] and the plasmid has been deposited with Addgene (http://www.addgene.org/Christopher_Marx, Plasmid 15670).References Herring CD, Glasner JD...
方法二:查找质粒图谱的网站:1.Vector Database(addgene)地址:http://www.addgene.org/这个网站很页面很人性化,直入主题,比如查找pRS类质粒图谱(注意,是一类质粒图谱,没关系,照样能找到),直接在搜索框输入pRS,可以看到,之类质粒一共有三十多个。2.Vector DB网址:http://genome-www.stanford.edu/vectordb/vecto...
We designed 4-6 gRNAs for each gene using the online tools available at http://sam.genome-engineering.org/database/ (Supplementary Table S1), generated activation plasmids and tested their activity in HEK293 and HeLa cells (Fig. 1, Supplementary Fig. S1a). Two days after transfection ...
The strong Th epitopes derived from cytomegalovirus (CMV) and influenza (Flu) virus (see Table 4) were obtained from an immune epitope database (IEDB, http://www.iedb.org), wherein the strong Th epitopes of CMV include pp65-11, pp65-71, pp65-92, pp65-123, pp65-128, pp65-57, pp65...