We thank Hope center at WUSM in St. Louis, MO, for providing machines, including Nanozoomer and flow cytometer, and for the lentivirus package service performed by Dr. Mingjie Li's group. Finally, we thank. Dr. Hemmo Meyer from the University of Duisburg-Essen for the VCP mcherry vectors...
5-FU was purchased from Sigma-Aldrich (St. Louis, MO). Lentiviral infection Human lenti-VCP, lenti-shVCP, lenti-vector control, and lenti-shNC were designed and purchased from GeneChem Technologies (Shanghai, China). The transfection was performed according to standard procedures. Following ...
(St. Louis, MO, USA); PP242 and Torin1 from Selleckchem (Houston, TX 77014, USA); TRAIL from KOMA BIOTECH (Seoul, South Korea); MitoTracker-Red (MTR), tetramethylrhodamine methyl ester (TMRM), 4′,6-diamidino-2-phenylindole (DAPI), and propidium iodide (PI) from Molecular Probes (...
All chemicals used for organic synthesis were purchased from Aldrich-Sigma (St. Louis, MO, USA). MitoTracker and Fura-2-AM were purchased from Invitrogen (Carlsbad, CA, USA). Protein A-agarose; antibodies against b-tubulin, b-actin, CCT-b, cytochrome c and Hsp90; siRNA for VCP; and ...
Louis, US Sigma-Aldrich, St. Louis, US NaOH Merck, Darmstadt, Germany Compounds α-Tocopherol (75 µM; 1 h) Bongkrekic acid (10 µM; 1 h) Carbachol (15 µM, 10.5 min) Cyclosporin A (1 µM and 10 µM; 1 h) FK506 (10 nM and 25 µ...
MATERIALS AND METHODS Cell lines and cell culture Parental and resistant cells from OVSAHO cell were cultured in RPMI 1640 (Caisson Labs, Smithfield, UT, USA, RPL03) with 10% fetal bovine serum (Sigma-Aldrich, St. Louis, MO, USA, F0926) with 1% penicillin–streptomycin solution (Caisson ...
The VCP/p97 inhibitor N2,N4-dibenzylquinazoline-2,4-diamine (DBeQ), was obtained from Sigma-Aldrich (St. Louis, MO). The proteasome inhibitor MG132 was purchased from EMB Millipore (Billerica, MA). Anti-FLAG M2 and anti-Myc-agarose affinity gels were purchased from Sigma-Aldrich. Anti-VC...