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TFEB (1:3000, Bethyl Laboratory303-673A), MFN2 (1:2000, Abcam#56889), S409/410 p-TDP-43 (1:2500, Cosmo Bio USA: CAC-TIP-PTD-M01). Goat anti-mouse horseradish peroxidase (HRP) or goat anti-rabbit HRP (1
One mg of lysate was incubated with 0.2 μl antibodies (Anti-VCP, Abcam ab11433 or Anti-FLAG, Sigma F1804) at 4 °C with rotation for the specified times. The reactions were then supplemented with 20 μl of Protein A/G magnetic beads (Thermo Scientific, 88802) and incubated for 5 ...
Antibodies for VDAC (ab14734, 1:2,000), Clpp (ab124822, 1:1,000), UBXD1 (ab103651, 1:500) and VCP (ab109240, 1:10,000) were from Abcam. EEA1 (3288, 1:500) and LC3 (2775, 1:1,000) antibodies were from Cell Signalling, WFS1 (NB100-1918, 1:1,000) antibody was from ...
(Farmingdale, NY, USA); poly (ADP-ribose) polymerase (PARP; ab32071) and Bap31 (ab37120) from Abcam (Cambridge, UK); Ras (clone RAS10, #05-516) from Millipore; The secondary antibodies used were anti-rabbit IgG HRP (G-21234) and anti-mouse IgG HRP (G-21040) from Molecular ...
VCP 昆虫 全长 N-His 100 ug 5500 抗体购买 产品名称 品牌 价格 VCP抗体/ VCP抗体 abcam 询价 VCP抗体/ VCP抗体 CST 询价 VCP抗体/ VCP抗体 Santa Cruz 询价 产品说明书pdf版和相关资料下载 产品应用举例 上一篇:GATAD2A基因GATAD2A蛋白 下一篇:NUP98基因Nup98-Nup96蛋白 分享到: 推荐7 全部...
The primary antibodies: VCP, Ki-67, E-cadherin, N-cadherin, and vimentin were purchased from Abcam (Cambridge, MA). Cyclin D1, CDK4, cyclin E, CDK2, PCNA, Bcl-2, Bax, Bcl-xL, and actin were purchased from Santa Cruz Biotechnology (Dallas, Texas). STAT3, p-STAT3 (Tyr705), p21,...
The sources of antibodies used in this study are as follows: monoclonal anti-FLAG antibody from Sigma; anti-VCP, anti-Npl4, and anti-Ufd1 antibodies from Abcam (Cambridge, UK); anti-ubiquitin antibody from Chemicon International; anti-HA antibody, anti-FAF1, and anti-GAPDH antibodies from ...
Calcineurin activity assay Calcineurin activity was measured using the cellular calcineurin phosphate activity assay (ab139464, Abcam, Cambridge, UK). Cells were plated 48 h prior to sample preparation in 10 cm2 dishes to reach 90% confluence. Cells were collected in 15 ml Tris-Buffered ...
(1:10,000, abcam, ab109390), β-actin (1:10,000, Santa Cruz, sc-47778). The blots were developed by SuperSignal West Pico PLUS or West femto PLUS Chemiluminescent Substrate (ThermoFisher). The luminescence signals were imaged by ImageQuantTM LAS4000 machine. Data were normalized over the ...