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Antibodies for VDAC (ab14734, 1:2,000), Clpp (ab124822, 1:1,000), UBXD1 (ab103651, 1:500) and VCP (ab109240, 1:10,000) were from Abcam. EEA1 (3288, 1:500) and LC3 (2775, 1:1,000) antibodies were from Cell Signalling, WFS1 (NB100-1918, 1:1,000) antibody was from ...
One mg of lysate was incubated with 0.2 μl antibodies (Anti-VCP, Abcam ab11433 or Anti-FLAG, Sigma F1804) at 4 °C with rotation for the specified times. The reactions were then supplemented with 20 μl of Protein A/G magnetic beads (Thermo Scientific, 88802) and incubated for 5 ...
Immunoprecipitation of VCP or CDC48 was performed with antibody to VCP (ab11433, Abcam) as described32. For western blotting, antibody to VCP (ab11433, Abcam), rabbit polyclonal antibody to trimethylated lysine (ab76118) (Abcam) and antibody to glyceraldehyde-3-phosphate dehydrogenase (Ambion) ...
(1:200), anti-Desmin rabbit antibody (1:200, Abcam#15200) at 4 °C overnight and followed by goat anti-rabbit Alexa Fluor-488 secondary antibodies (1:500) at room temperature for 1 h. All images were acquired with Keyence with identical configurations for all samples in the same ...
VCP Abcam ab109240; RRID:AB_10862588 Ub (P4D1) Santa-Cruz Biotechnology sc-8017; RRID:AB_628423 p62/SQSTM1 Proteintech 18420-1-AP; RRID:AB_10694431 HSPB1 Proteintech 18284-1-AP; RRID:AB_2295540 Normal Rabbit IgG Cell Signaling 2729S; RRID:AB_1031062 Myc-Tag (9B11) Cell Signaling 2276...
The primary antibodies: VCP, Ki-67, E-cadherin, N-cadherin, and vimentin were purchased from Abcam (Cambridge, MA). Cyclin D1, CDK4, cyclin E, CDK2, PCNA, Bcl-2, Bax, Bcl-xL, and actin were purchased from Santa Cruz Biotechnology (Dallas, Texas). STAT3, p-STAT3 (Tyr705), p21,...
VCP 昆虫 全长 N-His 100 ug 5500 抗体购买 产品名称 品牌 价格 VCP抗体/ VCP抗体 abcam 询价 VCP抗体/ VCP抗体 CST 询价 VCP抗体/ VCP抗体 Santa Cruz 询价 产品说明书pdf版和相关资料下载 产品应用举例 上一篇:GATAD2A基因GATAD2A蛋白 下一篇:NUP98基因Nup98-Nup96蛋白 分享到: 推荐7 全部...
Calcineurin activity assay Calcineurin activity was measured using the cellular calcineurin phosphate activity assay (ab139464, Abcam, Cambridge, UK). Cells were plated 48 h prior to sample preparation in 10 cm2 dishes to reach 90% confluence. Cells were collected in 15 ml Tris-Buffered ...
代理并销售进口SIGMA试剂、abcam抗体、R&D抗体、CST抗体、ATCC细胞、BD公司、GE公司、赛默飞世尔公司产品。上海博湖生物科技有限公司(Shanghai Bohu Biological Technology Co., Ltd.,China)先后与天津中医学院、复旦大学、上海交通大学医学院、上海交通大学、华东师范大学、第二军医大学、南京大学,暨南大学,南京工业大学,...