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TFEB (1:3000, Bethyl Laboratory303-673A), MFN2 (1:2000, Abcam#56889), S409/410 p-TDP-43 (1:2500, Cosmo Bio USA: CAC-TIP-PTD-M01). Goat anti-mouse horseradish peroxidase (HRP) or goat anti-rabbit HRP (1
p97, also known as valosin-containing protein (VCP) and Cdc48, is an evolutionarily conserved ATPase associated with various cellular activities, many of which are related to protein homeostasis or proteostasis1. In general, p97/VCP functions as an ATP-dependent segregase, extracting client prote...
Anti-VCP/p97, anti-UFD1, anti-NPL4, anti-γH2AX, anti-ubiquitin FK2, anti-Myc, and the antibodies against RPN2/S1 and Sug1 were purchased from Abcam, BD Biosciences, Abnova/Novus Biologicals (Littleton, CO), Cell Signaling Technology (Danvers, MA), EMB Millipore, Santa Cruz ...
The sources of antibodies used in this study are as follows: monoclonal anti-FLAG antibody from Sigma; anti-VCP, anti-Npl4, and anti-Ufd1 antibodies from Abcam (Cambridge, UK); anti-ubiquitin antibody from Chemicon International; anti-HA antibody, anti-FAF1, and anti-GAPDH antibodies from ...
Branched ubiquitin (Ub) chains constitute a sizable fraction of Ub polymers in human cells. Despite their abundance, our understanding of branched Ub function in cell signaling has been stunted by the absence of accessible methods and tools. Here we identify cellular branched-chain-specific binding ...
Western blots were performed according to the standard SDS-PAGE protocol with primary antibodies: AT8 (1:1000, Invitrogen, MN1020), S396 (1:10,000, abcam, ab109390), β-actin (1:10,000, Santa Cruz, sc-47778). The blots were developed by SuperSignal West Pico PLUS or West femto PLUS...
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Calcineurin activity assay Calcineurin activity was measured using the cellular calcineurin phosphate activity assay (ab139464, Abcam, Cambridge, UK). Cells were plated 48 h prior to sample preparation in 10 cm2 dishes to reach 90% confluence. Cells were collected in 15 ml Tris-Buffered ...
VCP 昆虫 全长 N-His 100 ug 5500 抗体购买 产品名称 品牌 价格 VCP抗体/ VCP抗体 abcam 询价 VCP抗体/ VCP抗体 CST 询价 VCP抗体/ VCP抗体 Santa Cruz 询价 产品说明书pdf版和相关资料下载 产品应用举例 上一篇:GATAD2A基因GATAD2A蛋白 下一篇:NUP98基因Nup98-Nup96蛋白 分享到: 推荐7 全部...