使用针对VCAM-1(5µg / mL,SoutherBiotech,英国),γH2AX(2µg / mL,法国Abcam,法国),Ki67(0.35µg / mL,法国达科),CD31(5µg / mL,法国PBBioscience),PDGFRβ(2µg / mL,法国圣克鲁斯),CD68(1µg / mL,法国默克密理博),...
产品概述 Abcam s VCAM1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VCAM1 in serum, plasma and cell culture supernatants.This assay employs an antibody specific for Human VCAM1 coated on a 96-well...
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Peritoneal mesothelial cells from both groups were then assayed for the presence of VCAM-1 (Abcam). The endometrial samples were assayed for a4尾1 integrin using flow cytometry. A Fisher's exact test was used to assess differences between groups. Results A total of 16 women were enrolled, ...
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VCAM-1 ELISA assay for murine and human plasma samples and cell culture supernatant were performed using DuoSet ELISA Development kit (R&D Systems, Minneapolis, MN, USA) and Mouse VCAM-1 ELISA kit (Abcam) according to the manufacturer’s protocol. TACE activity assay Activity of ADAM-17 was ...
Solid phase sandwich ELISA was used to quantify the concentration of VCAM1 target protein using a commercially available kit (Abcam® VCAM1 (CD106) Human ELISA Kit (ab46118)). ELISA was carried out according to the manufacturers protocol. Whole serum samples were diluted 1:50 prior to use....
用人TNF-α(20ng/ml,abcam,ab9642)即VCAM-1表达诱导因子处理细胞,并诱导VCAM-1表达14～16hrs。利用传代1～5的HUVEC细胞。 [0155]将人前单核细胞白细胞 (U937,CRL-1593.2TM)用 1% FBS/RPMI 1640(Gibco,22400)培养基洗涤两次(在1100rpm下离心3min),然后悬浮于人血清(Sigma,H4522)至5×10 个细胞 /ml的...