Summary: Cre transgenic mice can be used to delete gene sequences flanked by loxP sites in specific somatic tissues. We have generated vavCre transgenic mice, which can be used to inactivate genes specifically in adult hematopoietic and endothelial cells. In these animals, a Cre transgene is exp...
Conclusion YFP marked NK cells through Vav-Cre induced YFP reporter system in mice have high specificity and efficiency.%目的:探索Vav-Cre介导的黄色荧光蛋白(YFP)报告基因系统标记小鼠体内自然杀伤(NK)细胞的特异性和效率。方法通过基因型分型筛选ROSA26R-YFP与Vav-Cre小鼠杂交后代中双阳性基因型小鼠,流式...
Conclusion YFP marked NK cells through Vav-Cre induced YFP reporter system in mice have high specificity and efficiency.%目的:探索Vav-Cre介导的黄色荧光蛋白(YFP)报告基因系统标记小鼠体内自然杀伤(NK)细胞的特异性和效率。方法通过基因型分型筛选ROSA26R-YFP与Vav-Cre小鼠杂交后代中双阳性基因型小鼠,流式...
(2002). VavCre transgenic mice: a tool for mutagenesis in hematopoietic and endothelial lineages. Genesis, 34(4), 251-256. 这篇文章详细描述了Vav1-Cre小鼠的生成及其在研究造血和内皮细胞中的应用。 3. García-Flores, C., Medina, L., Rodríguez-Aguilar, L., Corbí, A. L., Hernández-...
(NK)cellsthrough Vav-CreinducedYFPreportersysteminmice.Methods ROSA26R—YFPandVav—Cremicewerecrossed.andtheirYFP andCregenedoublepositiveprogenywerescreenedbygenotyping.ThespecificityofYFPinhematopoieticcellsfromim— muneorgansincludinglymphnodes,spleen,thymusandbonema~owwereanalyzedbyflowcytometry.Thepercentages...
spleenandbonema~owwerealsoanalyzedbyflowcytometry.Results A totalof11doublepositivemice(ROSA26R—YFP一(+/一)VavCre)wereobtainedin17mouseoffspringbycrossingROSA26R— YFPmicewithVav—Cremice.ThepercentagesofYFPpositivecellsinimmuneorgansincludinglymphnodes,spleen,thy- BUSandbonemarrowwere73.87%±1.51%,56....
Cre mRNA is present in the blood plasma of Vav-iCre mice and contained in EVs including exosomes.Kirsten, RidderSascha, KellerMaria, DamsAnneKathleen, RuppJessica, SchlaudraffDomenico, Del TurcoJulia, StarmannJadranka, MacasDarja, Karpova
Conclusion Vav1 Cre strain can be efficiently recombined in macrophages(>90%)when crossing with a conditional reporter stain expressing YFP from the ubiquitously active Rosa26 locus(ROSA26R-YFP).[Key words ] macrophages;reporter mice;immune system;hybridization;fluorescent protein;Cre recombinase;geno-...
将2A-Cre插入到小鼠Vav1基因终止密码子处。 *使用本品系发表的文献需注明: Vav1-2A-Cre mice (Cat. NO. NM-KI-233228) were purchased from Shanghai Model Organisms Center, Inc.. 验证数据 Fig1. Cre mediated recombination in spleen of Vav1Cre/+; Rosa26tdTomato/+mouse. ...
Extended Data Fig. 2: Vav-iCre+/−;Ercc1−/fl mice maintain normal weight and body composition. From: An aged immune system drives senescence and ageing of solid organs a, Body weight (BW) of three different age groups of mice by genotype. b–d, Percentage fat (b), lean mass (...