基因名 Vav1 基因曾用名 Vav; vav-T 染色体位置 17 NCBI ID 22324 MGI ID 98923 Ensembl ID ENSMUSG00000034116 Pubmed Vav1 人类同源基因 VAV1 品系描述 The 2A-Cre expression cassette was knocked into the Vav1 gene stop codon site. *使用本品系
(2002). VavCre transgenic mice: a tool for mutagenesis in hematopoietic and endothelial lineages. Genesis, 34(4), 251-256. 这篇文章详细描述了Vav1-Cre小鼠的生成及其在研究造血和内皮细胞中的应用。 3. García-Flores, C., Medina, L., Rodríguez-Aguilar, L., Corbí, A. L., Hernández-...
0.29%±0.O3%.P<0.01).TherewerenoYFPexpressionsinnon-immuneorgansindoublepositivemiceandinnegative contro1mice(0.72%±0.43%ys0.92%±0.27%,P>0.o5).ThepositiveratesofYFPweresignificantlyhigherinNKcellsin lymphnodes,spleenandbonemarow(76.94%±0.84%、81.66%±1.18%and88. ...
Conclusion YFP marked NK cells through Vav-Cre induced YFP reporter system in mice have high specificity and efficiency.%目的:探索Vav-Cre介导的黄色荧光蛋白(YFP)报告基因系统标记小鼠体内自然杀伤(NK)细胞的特异性和效率。方法通过基因型分型筛选ROSA26R-YFP与Vav-Cre小鼠杂交后代中双阳性基因型小鼠,流式...
Summary: Cre transgenic mice can be used to delete gene sequences flanked by loxP sites in specific somatic tissues. We have generated vavCre transgenic mice, which can be used to inactivate genes specifically in adult hematopoietic and endothelial cells. In these animals, a Cre transgene is exp...
spleenandbonema~owwerealsoanalyzedbyflowcytometry.Results A totalof11doublepositivemice(ROSA26R—YFP一(+/一)VavCre)wereobtainedin17mouseoffspringbycrossingROSA26R— YFPmicewithVav—Cremice.ThepercentagesofYFPpositivecellsinimmuneorgansincludinglymphnodes,spleen,thy- BUSandbonemarrowwere73.87%±1.51%,56....
In VavCre 129 GD mice, glucocerebrosidase activity in the spleen was severely reduced, leading to GlcCer and GlcSph accumulation and Gaucher cell infiltration in the spleen and femurs. Transcriptomic analysis identified upregulation of inflammatory and lysosomal pathways. Immune cell deconvolution from ...
Conclusion Vav1 Cre strain can be efficiently recombined in macrophages(>90%)when crossing with a conditional reporter stain expressing YFP from the ubiquitously active Rosa26 locus(ROSA26R-YFP).[Key words ] macrophages;reporter mice;immune system;hybridization;fluorescent protein;Cre recombinase;geno-...
Hemosiderin granulosa cells can be seen more easily in the spleens of CKO mice. What’s more, CKO mice had stable transcription characteristics. In conclusion, our mouse model has stable GPI-deficient and mild hemolysis, which may be an ideal in vivo experimental model for PNH....
将2A-Cre插入到小鼠Vav1基因终止密码子处。 *使用本品系发表的文献需注明: Vav1-2A-Cre mice (Cat. NO. NM-KI-233228) were purchased from Shanghai Model Organisms Center, Inc.. 验证数据 Fig1. Cre mediated recombination in spleen of Vav1Cre/+; Rosa26tdTomato/+mouse. ...