1. de Boer, J., Williams, A., Skavdis, G., Harker, N., Coles, M., Tolaini, M., … & Kioussis, D. (2003). Transgenic mice with hematopoietic and lymphoid specific expression of Cre. European Journal of Immunology, 33(2), 314-325. ...
spleenandbonema~owwerealsoanalyzedbyflowcytometry.Results A totalof11doublepositivemice(ROSA26R—YFP一(+/一)VavCre)wereobtainedin17mouseoffspringbycrossingROSA26R— YFPmicewithVav—Cremice.ThepercentagesofYFPpositivecellsinimmuneorgansincludinglymphnodes,spleen,thy- BUSandbonemarrowwere73.87%±1.51%,56....
0.29%±0.O3%.P<0.01).TherewerenoYFPexpressionsinnon-immuneorgansindoublepositivemiceandinnegative contro1mice(0.72%±0.43%ys0.92%±0.27%,P>0.o5).ThepositiveratesofYFPweresignificantlyhigherinNKcellsin lymphnodes,spleenandbonemarow(76.94%±0.84%、81.66%±1.18%and88. ...
将2A-CreERT2-WPRE-pA插入到小鼠Vav1基因终止密码子处。 *使用本品系发表的文献需注明: Vav1-2A-CreERT2 mice (Cat. NO. NM-KI-233229) were purchased from Shanghai Model Organisms Center, Inc.. 验证数据 Fig1. Cre mediated recombination in spleen of Vav1CreERT2/+; Rosa26tdTomato/+ mouse...
Conclusion YFP marked NK cells through Vav-Cre induced YFP reporter system in mice have high specificity and efficiency.%目的:探索Vav-Cre介导的黄色荧光蛋白(YFP)报告基因系统标记小鼠体内自然杀伤(NK)细胞的特异性和效率。方法通过基因型分型筛选ROSA26R-YFP与Vav-Cre小鼠杂交后代中双阳性基因型小鼠,流式...
Summary: Cre transgenic mice can be used to delete gene sequences flanked by loxP sites in specific somatic tissues. We have generated vavCre transgenic mice, which can be used to inactivate genes specifically in adult hematopoietic and endothelial cells. In these animals, a Cre transgene is exp...
Objective Vav-Cre induced YFP reporter system in mice. Methods ROSA26R-YFP and Vav-Cre mice were crossed, and their YFPand Cre gene double positive progeny were screened by genotyping. The specificity of YFP in hematopoietic cells from im-mune organs including lymph nodes, spleen, thymus and ...
Conclusion Vav1 Cre strain can be efficiently recombined in macrophages(>90%)when crossing with a conditional reporter stain expressing YFP from the ubiquitously active Rosa26 locus(ROSA26R-YFP).[Key words ] macrophages;reporter mice;immune system;hybridization;fluorescent protein;Cre recombinase;geno-...
Paroxysmal nocturnal hemoglobinuria is a clonal disease caused by PIG-A mutation of hematopoietic stem cells. At present, there is no suitable PNH animal model for basic research, therefore, it is urgent to establish a stable animal model. We constructed
Cre mRNA is present in the blood plasma of Vav-iCre mice and contained in EVs including exosomes.Kirsten, RidderSascha, KellerMaria, DamsAnneKathleen, RuppJessica, SchlaudraffDomenico, Del TurcoJulia, StarmannJadranka, MacasDarja, Karpova