【产品编号】:ATCC® PCS-100-030 【产品规格】:475ML 【中文名称】:ATCC Vascular Cell Basal液体培养基 【英文名称】:无 【CAS.编号】:无 【详细描述】: VascularCellBasalMedium(ATCC®PCS-100-030™) 产品名称:ATCCVascularCellBasal液体培养基 货号:ATCC®PCS-100-030 品牌:ATCC 规格:475ML 温度:4...
(Waltham, MA, USA) maintained in vascular cell basal medium (ATCC, Manassas, VA, USA) and used before to passage 7. Fetal bovine serum, phosphate-buffered saline (PBS), penicillin–streptomycin (PS) and 0.25% (w/v) trypsin/1 mM EDTA were all purchased from Invitrogen (Carlsbad, CA, ...
Vascular Cell Basal Medium ATCC Cat# PCS-100-030 70-kDa FITC-dextran Sigma-Aldrich Cat# FD70S Critical commercial assays DNA purification kit Macherey-Nagel Cat# 740609.50 MEGAscript™ T7 Transcription Kit Thermo Fisher Scientific Cat# AMB13345 MEGAclear™ Transcription Clean-Up Kit Thermo Fisher...
Cells were GeroScience (2022) 44:2863–2884 cultured in SmBM medium (Lonza, Switzerland) or vascular cell basal medium (ATCC, LGC, Poland) sup- plemented as defined by the manufacturer, and kept in humidified atmosphere (37 °C and 5% CO2 in the air). To obtain population of ...
Cell migration was towards a lower chamber with DMEM medium only, or with supernatant derived from non-treated (control) macrophages, and sisham- or siCECR1-treated macrophages. (f) Panel of fluorescent images of dsRed-marked pericytes that have migrated through the transwell setting in response...
MS1 mouse pancreas ECs (CRL-2279; ATCC) were cultured in DMEM medium supplemented with 5% FCS at 37 °C and 5% CO2. Cell adhesion assay For cell adhesion assays, 48-well tissue culture plates were either uncoated or coated with 0.1% gelatin or 10 μg/mL fibronectin in PBS at 4...
RAW macrophage cells (RAW 264.7 from the ATCC) were cultured in DMEM/F12 medium, 10% FCS, 2 mm l-glutamine, gentamicin (25 μg/mL). For Western blot and Northern blot studies, cells were grown in 25- and 175-cm2 cell culture flasks until 90% confluent. For other studies the cells...
hTERT-immortalized endothelial cell line isolated from a primary culture of neonatal foreskin microvascular endothelial cells of the dermis (TIME CRL4025™ from ATCC) were seeded at 10 000cell/cm2 and amplified for 2 passages in complete medium composed of: Vascular Cell Basal medium + Micro...
Type-II L-arginine:ureahydrolase, arginase-II (Arg-II), is shown to activate mechanistic target of rapamycin complex 1 (mTORC1) pathway and contributes to cell senescence and apoptosis. In an attempt to elucidate the underlying mechanism, we identified m
Cell culture and treatment Human aortic VSMCs (HA-VSMCs) were obtained from the American Type Culture Collection (ATCC, Manassas, VA) and cultured in Dulbecco’s modified high glucose medium (DMEM-HG) (Gibco, New York, USA) containing 10% fetal bovine serum (AusGeneX, Gold Coast, Australi...