Homologous genes for these transporters are also found in plant and mammalian genomes. The physiological significance in response to nitrogen starvation can now be discussed. 2008 IUBMB IUBMB Life, 60(8): 519-525, 2008 展开 关键词: vacuole vacuolar amino acid transporter vacuolar H+‐ATPase ...
Vacuolar Amino Acid Transporter Avt5p Is Responsible for Lithium Uptake in Schizosaccharomyces pombedoi:10.1271/bbb.100259The fission yeast Schizosaccharomyces pombe was sensitive to salinity; cell growth was stopped by 0.5 M NaCl and by 10 mM LiCl. The avt5 + gene encodes a vacuolar transporter ...
(PHT5) following the systematic nomenclature of PHT1–PHT4 Pi transporters inArabidopsis28,29. PHT5;1 was also named Vacuolar Phosphate Transporter 1 (VPT1) in a very recent article30. Our findings shed light on the crucial role of SPX-MFS in plant adaptation to Pi fluctuations and provide...
Synthesis and cloning of the PfVIT coding sequence. The 822 bp sequence (PlasmoDB gene ID: PF3D7_1223700) encoding the 273 amino acid residue PfVIT transporter from Plasmodium falciparum 3D7 was codon-optimised for expression in Escherichia coli (Supplementary Fig. S2) and synthesised...
such as Ste2p, or undergoing specialized processing (precursor of carboxypeptidase S; pCps1p), are sorted into these vesicles, while protein constituents of the limiting vacuolar membrane, such as the Vph1p subunit of the vacuolar H+-ATPase, or iron transporter Fth1p, are excluded from them...
the general amino acid transporter GAP1 (67)), and our analysis indicates that we also enriched for plasma membrane proteins targeted to the vacuole for proteolysis. This result is in agreement with the GPF fusion analysis showing fluorescent signals at both the vacuolar lumen and the plasma memb...
Protein tolerance to random amino acid change. (2004) Proc. Natl. Acad. Sci. USA, vol. 101, pp. 9205-9210. Barkla et al. Physiology of ion transport across the tonoplast of higher plants. (1996) Annu. Rev. Plant Physiol. Plant Mol. Biol. vol. 47, pp. 159-184. Rausch et al...
Previous studies have shown that the salt sensitivity of yeast mutants can be partially compensated by the heterologous expression of plant vacuolar transporter genes. Therefore, the functional complementation in yeast mutants can be used to explore the function of plant Na+/H+ antiporters [37]. In...
Although AtRMR1 and -2 have similar transmembrane domains (39% amino acid sequence identity), and the same predicted 23-residue length (alignment Figure 5), the two proteins have different localizations. The length of the TM alone can determine protein localization in plant cells [36] but by...
Transgene analysis revealed that TaVP overexpression improved plant growth under normal conditions and specifically under Pi and N deprivation stresses, high salinity, and drought. The improvement of growth of the transgenic plants was found to be closely related to elevated V-H+-PPase activities in...