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We find that the lever-arm swing generates a large proportion of the force at low load ( < 0.5 pN), resulting in 3 kBT of work. At high load (1.9 pN), however, the contribution of the Brownian search-and-catch increases to dominate, reaching 13 kBT of work. We ...
For the LIM domain replacement strategy, we either used serial overlap extension PCRs with degenerated primers or cloned a de novo-synthetized nucleotide sequence, coding for the LIM domain of interest but with a degenerated codons compared to the original one. The amino acid sequence of the ...
The Vκ primers are designed such that only O2 cRS are detected. Figure 4 Vκ cRS cleavage is detected in C57BL/6 bone marrow pre-B cells and 103/BCL2 cells. LM-PCR was conducted with and without ligase on DNA extracted from pre-B cells of C57BL/6 mice or from 103/BCL2 cells ...
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A Primer on American Labor Law is an accessible guide for non-specialists and labor lawyers - labor and management representatives, students and general pr... WB Gould - MIT Press, 被引量: 18发表: 0年 Enduring Principles and Current Crises in Constitutional Criminal Procedure Akhil Amar informs...
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In particular, for adults the mechanical threshold (force-grams) was measured in naive and CCI at day 3, 7 and 21 after neuropathy induction (Supplementary Fig. 16). As control, we used mice that underwent surgery without the sciatic nerve ligature (sham animals) and na¨ıve animals, ...
alleles in historic samples, with shorter allele lengths being more prevalent. Lower template quality may have resulted from DNA shearing with formalin fixation [101], leading to partial repeat amplification if primer sites were unavailable for binding [102]. However, our Micro-Checker tests and ...