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Cells are subsequently lysed in 0.1 ml lysis buffer (1% Triton-X-100, 15% Glycerol, 2 mm EDTA, 2 mm DTT, and 25 mm MgCl2 in Tris-phosphate buffer pH 7.8) after which the total protein concentration of the lysate is measured (Biorad, protein standard II). To determine marker gene ...
The sections were placed in solution (37 °C,100 μl goat serum +50 μl 10%TritonX-100 + 850 μl PBS) for 20 min. Then the sections were incubated with primary antibody (diluted with PBS,1:500) at 4 °C overnight and incubated with secondary antibodies (diluted ...
Such symptoms include cognitive, sensory or motor problems, depending on the extent and regions of the brain which are damaged. These symptoms include paralysis or loss of muscle movement, difficulty walking, talking or swallowing (including dysarthria, dysphagia, and aphasia), and defects in working...
Triton: Fix html not parsed in search results Profiles: Typo fixes in locale Mailmotor: Fix errors when list is empty or has no users in it Improvements: Core: Added a basic implementation of the symfony form collection class with working add and delete buttons Core: Status fields with the...
Even though no chloroplast band could be observed, the forisomes, despite the treatment with Triton, were found primarily within the membrane band (1). Example 2 Separation of Forisomes by Means of SDS Polyacrylamide Gel Electrophoresis 1. The forisomes were centrifuged in V medium for 10 ...
The supernatant was removed and 100 μl of a 20:1 isopropanol/triton-X100 solution was added to the wells. The plates were put on a 96-well shaker for 3-5 minutes to solubilize the precipitated staining. Absorbance was measured at 540 nm and at 690 nm (background). By this assay, ...
The supernatant was removed and 100 μl of a 20:1 isopropanol/triton-X100 solution was added to the wells. The plates were put on a 96-well shaker for 3-5 minutes to solubilize the precipitated staining. Absorbance was measured at 540 nm and at 690 nm (background). By this assay, ...