TheHPLC method, more laborious and expensive, was demonstrated to be more accurate, precise and faster (analytical range 0.125–16 μg/mL, accuracy between −2.48 and 3.70% and precision between 2.77 and 5.9
temperature and detection wavelength of 495 nm. The retention time of Z-GP-DOX was 6.6 min. A linear curve over the concentration range of 1-1200 g/mL (r2 0.999) was established, and the LOD and LOQ for Z-GP-DOX were 0.5 and 1 g/mL,...
55 No. 13 HPLC time-of-flight high-resolution mass spectrometry (HPLC-TOF/MS) techniques. Additionally, a material reference characteristic chromatographic method based on HPLC-UV-ELSD was established to precisely determine its similarity range and peak attribution of its characteristic chromatographic ...
It is commonly used for determining the concentration of a substance in a solution based on the Beer-Lambert law, with a wavelength range of 200-400 μm. UV absorption is a valuable technique in various applications such as drug analysis and HPLC detection....
Results The linear range of nictinic acid was 5. 0~100. 0 μg/mL. With UV method (r=0. 995 9),the average recovery is 99. 57%(RSD=1. 08%,n=6);with HPLC method (r=0. 999 8),the average recovery is 99. 62% (RSD=0. 74%,n=6).Conclusion The HPLC/UV method is simple...
The detector’s precise optical design ensures accurate and stable wavelength selection across this range, making it well-suited for routine quantitation, impurity profiling, and low-level analyte detection in both HPLC and UHPLC workflows. What is the benefit of dual-wavelength...
UV–visible spectroscopy is based on the measurement of the ratio of the passed light with respect to the incident light in the wavelength range from the UV to the visible. From:Nanotechnologies in Preventive and Regenerative Medicine,2018
Thelinearrangeoftriacylglycerolswas5.998~59.98g(r=0.9993).theaverage recoveryratewas (n=6)100.50% (RSD=I.307%).Conclusion:Asimple,accurateandreproducibleHPLC-UV methodwasestablishedfordeterminationtriacylglycerolsinCoixseed. Keywords:HPLC—UV ;coixseed;triacylglycerols;quantitation 薏苡仁为禾本科植物薏苡 ...
论文标题: HPLC-UV法测定大鼠脑组织中利培酮的含量 英文标题: 中文摘要: 目的:建立大鼠脑组织中利培酮含量的测定方法。方法:取大鼠,ig 30 mg/kg的利培酮混悬液,30 min后断头处死,分离脑组织;采用高效液相色谱-紫外法,以盐酸小檗碱为内标,测定大鼠脑组织中利培酮的浓度。色谱条件采用色谱柱Wondasil C18(250 mm×...
filtration was performed. Different concentrations inside the working range of each drug were prepared and the previously explained method in “Calibration graphs construction” section was followed. The content of each drug in the prepared dosage form was calculated using the corresponding regression equa...