Determination of the linear range is one of the main concerns in validation of an HPLC analysis method. It is particularly important since single point calibration will be then used routinely. We proposed an iterative methodology to handle this problem. The idea was, at each step, to test stat...
VIAL J., JARDY A., Study of the linear range in HPLC analyses with UV detection: Methodology and experi- mental application to the influence of the analyte UV- spectrum, Journal of High Resolution Chromatography, 1999, 22, 217-221.
论文标题: HPLC-UV法测定大鼠脑组织中利培酮的含量 英文标题: 中文摘要: 目的:建立大鼠脑组织中利培酮含量的测定方法。方法:取大鼠,ig 30 mg/kg的利培酮混悬液,30 min后断头处死,分离脑组织;采用高效液相色谱-紫外法,以盐酸小檗碱为内标,测定大鼠脑组织中利培酮的浓度。色谱条件采用色谱柱Wondasil C18(250 mm×...
This is complemented by advanced thermal wander management, which reduces baseline wander caused by ambient temperature fluctuations, thus maintaining stability and reproducibility in analytical results. The versatility of the 2489 UV/Vis Detector is further evidenced by its wide ra...
The calibration curves were linear within a concentration range of 0.7–8.4μgmL 1 ( r 2 =0.9994). The inter-day and intra-day accuracy and precision were ≤3.33 and ≤5.55%. The limit of detection (LOD) and limit of quantification (LOQ) were 0.35 and 0.7μgmL 1 . The average ...
Linearrange was17.9～89.0μg/mL,andrecoveryratewas99.6%(RSD=0.4%).Theaverageaccumulateddissolutionwas 92.3%(RSD=1.9%)at10min.ConclusionThetwomethodsareequivalentindeterminingdissolutionofazithro- mycintablet,andtheyaresimpleandaccuratewithgooddissolutioneffect. Keywords:Azithromycintablet;Dissolution;HPLC;UV ...
柱前衍生HPLC—UV法测定人工牛黄中猪去氧胆酸含量目的建立柱前衍生HPLC-UV法测定人工牛黄中猪去氧胆酸含量的方法。方法以2-萘基溴甲基酮为衍生试剂、三乙胺为催化剂、60℃水浴条件下对猪去氧胆酸进行衍生,采用HPLC-UV法测定人工牛黄中猪去氧胆酸含量。结果当衍生试剂与猪去氧胆酸的摩尔比>20∶1,催化剂与猪去氧...
5um)chromatographic column,Methanol -water(volume ratio of 65:35)as mobile phase. Flow rate:1. 0 ml / min,Detection at237 nm,sample volume for the 20 ul;by ultraviolet spectrophotometer,for the wavelength of 237 nm. Results HPLC:Nitrendipine Tablet has perfect linear correlation inthe range ...
Both assays were linear over the concentration range of 2–30 μg/mL (R≈0.999), as well as accurate and precise, with recoveries between 98 and 100% and relative standard deviation (%RSD) lt; 2.0%. The proposed methods are highly sensitive, precise and accurate and were successfully ...
140 mg?kg-1 SAMe was administrated by intravenous and plasma concentration were determined by HPLC-UV assay. Results: SAMe was successfully separated. The calibration curve showed a good linear regression within the range of 10~2 000 μg?mL-1. The regression equation was Y = 30241X +256204...