Offering both the superior sensitivity and the linear dynamic range needed for the detection of even the lowest-level impurities, ACQUITY UPLC and ACQUITY Premier TUV Detectors enable simultaneous quantitation of major and minor compounds in both routine and complex, ult...
论文标题: HPLC-UV法测定大鼠脑组织中利培酮的含量 英文标题: 中文摘要: 目的:建立大鼠脑组织中利培酮含量的测定方法。方法:取大鼠,ig 30 mg/kg的利培酮混悬液,30 min后断头处死,分离脑组织;采用高效液相色谱-紫外法,以盐酸小檗碱为内标,测定大鼠脑组织中利培酮的浓度。色谱条件采用色谱柱Wondasil C18(250 mm×...
(UV/Vis) Detector is the best detection choice for performance, reliability, and usability. Linear range of 2.5 AU enables the identification and quantitation of both major and minor components in the same run. Ability to monitor absorbance at two user-selectable wavelengths, resulting in more ...
55 No. 13 HPLC time-of-flight high-resolution mass spectrometry (HPLC-TOF/MS) techniques. Additionally, a material reference characteristic chromatographic method based on HPLC-UV-ELSD was established to precisely determine its similarity range and peak attribution of its characteristic chromatographic ...
The HPLC separation was performed on a Supelcosil LC-CN column, using a mobile phase consisting acetonitrile: 25 mM phosphate buffer at pH 3.5 (15: 85 v/v). The calibration curve is linear in the concentrations range of 25-1000 ng/mL, which is suitable for pharmacokin...
Walvelength range 200-1000nm Walvelength accuracy ±2nm Spectral bandwidth 3.8nm Stray light ≤0.5%(T) (at 220,360nm) Company Profile Wincom Company Ltd. is a prfessional leading enterprisespecializing the manufacturing and supply of Laboratoy Equipments ...
Determination of the linear range is one of the main concerns in validation of an HPLC analysis method. It is particularly important since single point calibration will be then used routinely. We proposed an iterative methodology to handle this problem. The idea was, at each step, to test stat...
Finally,the content range of Baicalin in Banxia Xiexin Decoction was309.23~574.28mg.CONCLUSION This method is effective and simple to determine the content of Baicalin in Banxia Xiexin Decoction,which lays a foundation for further research and development of Banxia Xiexin Decoction.Key words:B ...
Table 1. Regression data for HPLC determination of TA in aqueous methanol and in urine Regression data Calibration range (lg mL)1) Detection limit (lg mL)1) Quantitation limit (lg mL)1) Regression equationa slope (b) Standard deviation of the slope (Sb) Relative standard deviation of the ...
·min,1anddetectionwavelengthof220nmMobilephaseconsistedofacetonitrile-water,85∶15,,columntemperaturewassetatroomtemperatureandinjectionvolumewas5µLRESULTS,Excellentlinearcorrelationwasshowedwithintherangeof02,10mg·mL(1)r(09993)withrecoveryof985%(RSD)142%,,theminimumdetectionlimitwas30ng,S/N≥3,The...