Determination of the linear range is one of the main concerns in validation of an HPLC analysis method. It is particularly important since single point calibration will be then used routinely. We proposed an iterative methodology to handle this problem. The idea was, at each step, to test stat...
Jardy, Study of the linear range in HPLC analyses with UV detection: methodology and experimental application to the influence of the analyte UV spectrum, J. High Resolut. Chroma- togr. 4 (22) (1999) 217-221.Vial J, Jardy A. Study of the linear range in HPLC analyses with UV...
论文标题: HPLC-UV法测定大鼠脑组织中利培酮的含量 英文标题: 中文摘要: 目的:建立大鼠脑组织中利培酮含量的测定方法。方法:取大鼠,ig 30 mg/kg的利培酮混悬液,30 min后断头处死,分离脑组织;采用高效液相色谱-紫外法,以盐酸小檗碱为内标,测定大鼠脑组织中利培酮的浓度。色谱条件采用色谱柱Wondasil C18(250 mm×...
This is complemented by advanced thermal wander management, which reduces baseline wander caused by ambient temperature fluctuations, thus maintaining stability and reproducibility in analytical results. The versatility of the 2489 UV/Vis Detector is further evidenced by its wide rang...
The calibration curves were linear within a concentration range of 0.7–8.4μgmL 1 ( r 2 =0.9994). The inter-day and intra-day accuracy and precision were ≤3.33 and ≤5.55%. The limit of detection (LOD) and limit of quantification (LOQ) were 0.35 and 0.7μgmL 1 . The average ...
Linearrange was17.9～89.0μg/mL,andrecoveryratewas99.6%(RSD=0.4%).Theaverageaccumulateddissolutionwas 92.3%(RSD=1.9%)at10min.ConclusionThetwomethodsareequivalentindeterminingdissolutionofazithro- mycintablet,andtheyaresimpleandaccuratewithgooddissolutioneffect. Keywords:Azithromycintablet;Dissolution;HPLC;UV ...
·min,1anddetectionwavelengthof220nmMobilephaseconsistedofacetonitrile-water,85∶15,,columntemperaturewassetatroomtemperatureandinjectionvolumewas5µLRESULTS,Excellentlinearcorrelationwasshowedwithintherangeof·(1)(02)10mg·mL(1)r(09993)withrecoveryof985%(RSD)142%,,theminimumdetectionlimitwas30ng,S/N≥3...
5um)chromatographic column,Methanol -water(volume ratio of 65:35)as mobile phase. Flow rate:1. 0 ml / min,Detection at237 nm,sample volume for the 20 ul;by ultraviolet spectrophotometer,for the wavelength of 237 nm. Results HPLC:Nitrendipine Tablet has perfect linear correlation inthe range ...
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Offering both the superior sensitivity and the linear dynamic range needed for the detection of even the lowest-level impurities, ACQUITY UPLC and ACQUITY Premier TUV Detectors enable simultaneous quantitation of major and minor compounds in both routine and complex, ultr...