(n = 74) derived from cross-link data with SCML2 to extract precursor ion chromatograms (XIC). Picked precursors were examined manually for the presence of at least two isotope peaks and, when present, in a ± 2 min time window of measured retention times and anm/zmatch ...
using a modified RPLC column variant (ACE AQUA) for increased resolution of the polar analytes (Fig.2, 1D view; similar results were obtained using wild geese samples). Instant re-analysis of the isolated “compound X” revealed a pure peak, but the peak...
Fig. 2.The linear relationship between the peak area of SEC-chromatograms and the measured results of SRNOM andSRFAby bench-instruments: (a) the peak area of PDA-detector chromatogram and the UVA254measured by UV–vis spectrometer; (b) the peak area of fluorescence-detector chromatogram and ...
Absorbance units full-scale (AUFS) vary from 0.0001 to 4.000 AU. Note: Changing the sensitivity (AUFS) setting affects the 2-V output. • Chart polarity (+ or –) – Reverses the polarity of the charted chromatogram. Select + for a normal chromatogram, or – for an inverted chromatogram...
As HPLC usually represents a better method for quantitative measurements, we determined the protein purities based on the main peak percentage of the integrated LC-220 chromatograms. The LC-220 chromatograms of the six tested proteins are shown in Figure S5 (Electronic Supplementary Material). The...
Absorbance units full-scale (AUFS) vary from 0.0001 to 4.000 AU. Tip: Changing the sensitivity (AUFS) setting affects the 2-V output. • Chart polarity (+ or –) – Reverses the polarity of the charted chromatogram. Select + for a normal chromatogram, or – for an inverted chromatogram...
A representative chromatogram is shown in Figure 2A. No aggregates were observed in all the control samples, but after 24 h of UV-B treatment, significant levels of aggregation were observed in all the samples (Figure 2B), which was consistent with the results obtained from SDS-PAGE analysis...