coenzyme biosynthesis, nucleotide-binding domains related to the UspA protein (USPA domains), photolyases, electron transport flavoproteins, and PP-loop-containing ATPases together comprise a distinct class of alpha/beta domains designated the HUP domain after HIGH-signature proteins, UspA, and PP-...
Protein Expression and Purification, vol. 48, pp. 134-141 (2006). Yu-Ching, et al., “Impact of sequence diversity in the Moraxella catarrhalis UspA2/UspA2H head domain on vitronectin binding and antigenic variation”; Microbes & Infection 2013; pp. 375-387; vol. 15(5). Parent U.S...
catarrhalis UspA1 or UspA2 protein, polypeptide, domain, peptide or any fusion protein thereof. Herein are provided at least an isolated gene, DNA segment or coding region that encodes a M. catarrhalis uspA1 gene comprising about 2493 base pairs (bp) (SEQ ID NO:2) of strain O35E, about...
To purify the recombinant protein, each bacterial pellet was resuspended in 20 mM potassium phosphate buffer (pH 8.0), containing 500 mM arginine and a protease inhibitor cocktail. Cell lysis was performed prior to purification on immobilized metal affinity chromatography through NiNTA resin (Qiagen)...
Ubiquitous surface protein (UspA) 2 and its hybrid form UspA2H bind vitronectin at the highly diverse N-terminal head domain. Here we characterized the sequence diversity of the head domain in multiple M. catarrhalis clinical isolates (n = 51) with focus on binding of vitronectin. The ...
In one embodiment, the immunogenic composition comprises a protein of formula (I) wherein A is an immunogenic fragment of UspA2 that comprises a laminin binding domain and a fibronectin binding domain. In an additional embodiment, the immunogenic composition comprises a protein of formula (I) wher...