【蛋白质/抗原/多肽】 免疫球蛋白 封闭肽 人蛋白和抗原 小鼠蛋白和抗原 细菌蛋白和抗原 病毒蛋白和抗原 植物蛋白和抗原 其它蛋白和抗原 细胞质蛋白 总蛋白 膜蛋白 核蛋白 细胞裂解和提取物 线粒体蛋白 细胞裂解 组织提取 重组细胞因子 其它 【常用生化试剂】EDTA DTT Tris SDS MOPS HEPES 水 分子生物学缓冲液 ...
Finally, polyubiquitinated c-Myc was analyzed by SDS-PAGE gel and immunoblotting. In vitro deubiquitination assay HA-c-Myc and Myc-Ubiquitin plasmids were transfected into 293 T cells. 6 h before harvesting, 10 μM MG132 was added to treat cells. We lysed 293 T cells transfected ...
Equal amounts of protein per sample were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred onto a polyvinylidene fluoride membrane (Immobilon-P Transfer Membrane, Ireland). After blocked with 5% skim milk, the blots were incubated overnight with ...
For western blot analysis, 15–50 µg of cell lysates from each cell line with/without GNE-6776 or Ro-3306 treatment were loaded onto 8–12% SDS-PAGE gels and transferred onto nitrocellulose membranes (GE Healthcare Life Science, Bucks, UK). Membranes were incubated with antibodies over...
Check your price Request bulk or custom format Application Created with Sketch. FIGURE:1/2 USP16 Antibody (MA5-26205) in WB Western blot analysis of USP16 in HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12 and MCF7 cells using 35 µg per lane. Samples were separated by SDS-...
Search the latest USP Reference Standards Catalog and order online at www.usp.org.1 USING USP REFERENCE STANDARDS Uses of USP Reference Standards USP Reference Standards are integral components of mono-graphs and other documentary standards established by USP to help ensure the identity, strength, ...
For endogenous PD-1 ubiquitination assays, cells were treated with the proteasome inhibitor MG132 as indicated and were lysed in denaturing buffer (50 mM Tris, 1% SDS, 0.5 mM EDTA, and 1 mM dithiothreitol, pH 7.5). After incubation at 100 °C for 10 min, the cell lysates ...
All information provided in support of this product, including SDS and any product information leaflets have been developed and issued under the Authority of the issuing Pharmacopoeia.For further information and support please go to the website of the issuing Pharmacopoeia. Application Fenofibrate USP ...
9.Spill prevention: SOPs must be developed for spill prevention and containment, and all personnel required to clean up an HD spill must receive training in spill management, use of PPE, and NIOSH-certified CTSDs. The SOPs must address the size and the scope of the spill, who is responsibl...
Cells were washed with PBS and resuspended with lysis buffer (25 nM, pH 7.5 Tris-HCl, 150 mM NaCl, 5 mM EDTA, 1% Triton X-100, 1% SDS). Samples were sonicated (output level of 4, 15 s on and 15 s off, total 3 min) on ice to shear chromatin to an average ...