Total RNA was quantified using the Qubit™ RNA High Sensitivity (HS) and Broad Range (BR) Assay Kits (Thermo Fisher), further diluted to 2.5 ng/µL, and analyzed using a Bioanalyzer RNA 6000 pico assay to calculate the RNA integrity number (RIN) (See Supplementary Information) (Supple...
In this regime, signal-to-noise amplitude ratio should be nearly 10, to achieve a decent reconstruction. This SNR requirement would demand a sub-picotesla DC diamond NVC magnetometry, which has not been demonstrated yet. However, new techniques, with genetic manipulations for specific enhancement ...
To fill this gap, we develop a psychological cognitive framework composed of CP, OP, FoF, EE, and EM as interdependent configurations. We use this framework to explore how combinations of multiple psychological cognition shape FEA. The data analysis results supported the stance that CP, OP, FoF...
Ethics approval was granted by the Faculty of Medicine Ethics Committee, University of Southampton (Submission Number 32140) and the qualitative study was conducted to provide the ‘Outcomes’ and ‘Methodology’ of the PICO-M (Population, Intervention, Comparator and Outcomes Methodology) that shaped...
no attribute 'bool_' " problem after you set PYTHONPATH, you'd better check if you set too much different PATHONPATH via "echo $PYTHONPATH", if have more than one path, you can logout and log in, then add "expoet PYTHONPATH=$PYTHONPATH:/home/develop/caffe/python", then have a ...
I have been trying to develop a small program to test wifi on a suspect ESP-12S (ESP8266) but I have a preferences.h error on compilation using Arduino IDE 2.0.4 on Linux Kubuntu 22.04 . The program was put together from a tutorial by ‘Liz’ online: The error is” Detecting librarie...
An essential question in cancer is why individuals with the same disease have different clinical outcomes. Progress toward a more personalized medicine in cancer patients requires taking into account the underlying heterogeneity at different molecular le
While transcriptome- and proteome-wide technologies to assess processes in protein biogenesis are now widely available, we still lack global approaches to assay post-ribosomal biogenesis events, in particular those occurring in the eukaryotic secretory system. We here develop a method, SECRiFY, to sim...
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After quantification using PicoGreen (Molecular Probes), all PCR products were transferred to 384-well plates, lyophilized and re-suspended in 3 × SSC to a final concentration of 0.150 mg/ml. The spotting was done with a Chip WriterPro spotter (Virtek, Biorad) using PT3000 pins supplied ...