PLA was performed using the Duolink PLA technol- ogy (Sigma-Aldrich) following the manufacturer's protocol. Coverslips were affixed onto glass slides using ProLong Gold antifade mountant (Invitrogen) with DAPI.
Duolink PLA probe incubation Timing: ∼1.5 h This section describes the steps for probe hybridization. Note: Bring the wash buffer to room temperature (22°C–26°C) before use. Note: Do not allow the cells to dry. Make sufficient solution for all samples. Ligation Timing: 1 h This se...