The recovered pool is incubated with the control cell line to filter out the sequences that bind to common molecules on both the target and the control, leading to the enrichment of specific binders to the target. Binding sequences are amplified by PCR using fluorescein isothiocyanate–labeled se...
On the basis of the fact that cell-SELEX has the potential to develop aptamers for unknown molecules, as noted in (ii) above, these probes can be used through affinity precipita- tion to purify the target for aptamer recognition and then for disease biomarker discovery26,63,64. 4. Aptamers...
On the basis of the fact that cell-SELEX has the potential to develop aptamers for unknown molecules, as noted in (ii) above, these probes can be used through affinity precipitation to purify the target for aptamer recognition and then for disease biomarker discovery26,63,64. 4 Aptamers ...