Such focus-sensitive signal can be obtained, for instance, in confocal systems by using a pinhole21 or in structured illumination microscopy22, but in-plane scanning or multiple images per plane are needed, respectively. Alternatively, it is possible to achieve a focus-sensitive signal by simply ...
For those who can't get their hands on a pair of eclipse glasses, there is an alternative to the fancy specs: a DIY projection box. Child looks through a pinhole made from a cereal box to view the solar eclipse along the waterfront near the Children's Museum in Boston, Aug. 21...
The HeLa cells were prepared with the 3% glutaraldehyde fixation protocol and imaged with a sCMOS camera Zyla 4.2 from Andor using the Dragonfly Unit with a pinhole size of 40 µm. For each plane 15,000 frames with a frame rate of 5 Hz and a readout bandwidth of 540 MHz at...
This work demonstrates and guides how to use a range of state-of-the-art artificial neural-networks to analyse bacterial microscopy images using the recently developed ZeroCostDL4Mic platform. We generated a database of image datasets used to train networks for various image analysis tasks and pr...
(window width of 14 keV) for ~50 min with 20 projections using the highest-sensitivity 2 mm pinhole collimators (nine holes per head). The field of view on the nanoSPECT is extended using a step-and-shoot helical scan of the animal, with the user defining a range from 24 to ...
Since such large area mosaic acquisition takes time, z stack imaging of the 10 µm-thick tissue sections was avoided by adjusting the pinhole size to an equivalent of 4.63 airy units. The pinhole adjustments allowed acquisition of image information above and below the focal plane. All ...
A single optical fiber was operated as the illumination point source and the detection pinhole. A solid-state laser delivered an excitation wavelength of 488 nm at a maximum laser power output of 1 mW or lower at the tissue surface. Confocal image data were collected with 1100-fold ...