The invention further provides compositions and methods that provide an internal standard for the validation of the amplification of DNA. The invention is based on the use of hybrid oligonucleotides that have two domains that are specific for two genetically distinct regions of DNA, e.g., in two...
DNA amplification using primer pairs (Supplementary Fig. 3) was done in the presence of the SensiMix Sybrgreen reagents, all according to manufacturer instructions. Technical replicates were averaged and normalized to housekeeping genes GUS. The results shown are representative of at least three ...
The invention further provides methods of making variants and fragments thereof, such antigens and antigens from species of Chlamydia, as well as methods for producing a cell expressing such antigens. Specifically, whether produced by (... 被引量: 0发表: 0年 Coamplification of target nucleic ...
[0049] The DNA template may be amplified using any of a variety of DNA amplification methods. In some embodiments, the amplification of the DNA template may be performed using thermal cycling methods, such as polymerase chain reaction (PCR). In some embodiments, the DNA template may be ...
A set of universal primers for amplification of polymorphic non-coding regions of mitochondrial and chloroplast DNA in plantsdoi:10.1111/j.1365-294X.1995... B Demesure,N Sodzi,RJ Petit - 《Molecular Ecology》 被引量: 2481发表: 1995年 Chloroplast microsatellites: new tools for studies in plan...
(Fig.2a). This pattern was consistent even after excluding thymic samples to account for developing T cells (Extended Data Fig.2a). These nonproductive contigs withoutVgenes were captured in scVDJ-seq because the rapid amplification of 5′ complementary DNA (cDNA) ends (5′ RACE) technology ...
thaliana development has been demonstrated through the investigation of the AtSDC deficient mutant. A T-DNA insertion in the single A. thaliana SDC gene showed developmental defects, including necrotic leaf lesions, multiple inflorescences and flower sterility [7]. The functional characterization of ...
Methods for preparing cells that contain artificial chromosomes, methods for preparation of artificial chromosomes, methods for purification of artificial chromosomes, methods for targeted insertion of heterologous DNA into artificial chromosomes, and methods for delivery of the chromosomes to selected cells ...
DNA polymerase activity, an enzyme having DNA dependent DNA polymerase activity, an enzyme having Rnase H activity, an enzyme having RNA polymerase activity, and-maintaining the thus created reaction mixture under the appropriate conditions to a sufficient amount of time for the amplification to take...
[0029] A polymerase chain reaction was carried out as follows: the genomic DNA was amplified in eight parts specific for the PON1-gene and for its exons 1 to 9.Eight different amplifications were made, with eight different PCR primer pairs (SEQ ID NO:5-20); one pair for each exon exc...