a specialized continuous centrifuge. Research on post-harvest preservation is required to extend shelf-life beyond 4 to 8 weeks, and also to prepare concentrates from flagellate species (e.g. Isochrysis sp. and P. lutheri). Alternative processes have meanwhile been developed that are potentially ...
The whole broth was clarified (Sharples centrifuge). The pH of the clarified broth was adjusted to pH 9.0 by addition of NaOH 10% and extracted with 25 litres of ethyl acetate. After 20′ mixing, the two phases were separated. The organic phase was frozen overnight and then, filtered for...
ml conical centrifuge tube. The portion of the support that is contacted with the sample of feces can be removed from the rest of the support in order to achieve a convenient size. The portion of the support that includes the dried sample of feces is then washed with an aqueous solution....
Cells were centrifuged with a Sorvall Legend RT tabletop centrifuge at 4000 rpm for 10 minutes at 25° C. Cloning was performed with a TECHNE TC-312 thermocycler. All restrictive enzymes were purchased or provided pro gratis from Promega (Madison, Wis.). Scintillation fluid was PerkinElmer ...
Bacteria were then pelleted at 3,000 rpm for 5 minutes in a Sorvall RT6000B centrifuge (Sorvall Instruments, Newton, Conn.). 5 mL of 10 mM sodium phosphate buffer (pH 7.4) was added and the preparation was re-pelleted. The supernatant was decanted and 5 mL of fresh buffer was added...
The present invention relates to rapid, reliable and effective assays for screening and identifying pharmaceutically effective compounds that specifically inhibit the biological activity of fungal GTPase proteins, particularly GTPases involved in cell wall integrity, hyphael formation, and/or other cellular...
Examples include microtitre plates, test tubes, and micro-centrifuge tubes. In one embodiment, a fusion protein can be provided which adds a domain that allows the protein to be bound to a matrix. To illustrate, glutathione-S-transferase/p16 (GST/p16) fusion proteins can be adsorbed onto ...
Cells were harvested when 100% of the cells were infected with ehrlichiae and were then pelleted in a centrifuge at 17,000×g for 20 min. Cell pellets were disrupted with a Braun-Sonic 2000 sonicator twice at 40 W for 30 sec on ice. Ehrlichiae were purified as described previously (...
Centrifuge as above and discard supernatant. 5. Repeat step 4 with 25 ml cold water. 6. Resuspend in 2 ml of ice-cold sterile 1 M sorbitol. Centrifuge as above and discard supernatant. 7. Resuspend in 50 ml ice-cold 1 M sorbitol. Final volume of cells is about 150 ml (enough ...
The sample was centrifuged at 1,000 g on a swinging bucket centrifuge for 15 min at 4° C. One mL aliquots of the supernatant were transferred to microfuge tubes and spun at maximum speed in a microfuge to pellet down any remaining debris. The supernatants were collected and stored frozen ...