surfactant/detergent such as N-lauroyl sarcosine, a non-ionic surfactant/detergent such as Triton X-100, optionally a buffering agent such as Sodium Acetate at acidic pH such as pH 4.5, and optionally a reducing agent such as Dithiothreitol (DTT), and to methods of using such a composition....
Of note, the line width of an NMR signal is related to the molecular weight of the protein tumbling in solution. Thus, the line broadening observed during complex formation will reflect the increase in the molecular weight of the complex. In addition, the stability of the complex, that is,...
Double membrane vesicles (DMVs) serve as replication organelles of plus-strand RNA viruses such as hepatitis C virus (HCV) and SARS-CoV-2. Viral DMVs are morphologically analogous to DMVs formed during autophagy, but lipids driving their biogenesis are l
In the presence of thiols such as DTT or cysteine, the linker region undergoes specific self-cleavage which releases the target protein from the chitin-bound chitin-binding domain. These current protein purification systems have some disadvantages. The purification processes are inconvenient and ...
Purity/Purification> 98% by SDS-PAGE Form/FormatLiquid. In 50 mM Tris-HCl Buffer (pH 8.0) containing 0.5 mM DTT 10% glycerol Concentration1 mg/ml (determined by Bradford assay) (lot specific) Antigen SpeciesHuman Endotoxin Level< 1.0 EU per 1 microgram of protein (determined by LAL method...
ostreolysin A (OlyA) sterol regulatory element-binding protein (SREBP) Introduction The cholesterol content of the plasma membranes (PMs)5 of animal cells is tightly regulated by a network of proteins that reside in membranes of the endoplasmic reticulum (ER) (1). When PM cholesterol levels devi...
1a). Considering the high cellular heterogeneity of the human striatum, we carried out ATAC-seq on FANS-sorted neuronal and non-neuronal (mainly glial) cells based on the presence or absence of nuclear marker NeuN (feminizing locus on X-3), a nuclear protein specific to neurons in ...
Dynamic interactions of neurons and glia in the ventral midbrain mediate reward and addiction behavior. We studied gene expression in 212,713 ventral midbrain single nuclei from 95 individuals with history of opioid misuse, and individuals without drug e
The present invention generally relates to the production of antigen-specific T regulatory cells (Tregs). Such cells can be used in therapy to minimize undesirable immune responses
PITC Edman reagent is injected as 5% PITC, 5% TEAFTA (triethylammonium trifluoroacetate) and 0.01% DTT in butyl chloride. The sample electrode is charged negatively, with both proximal electrodes 128, 130 positive, 15 volts 0.6 μA to achieve coupling of the PITC with the N-terminal amino ...