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We created this guide to give you an idea of what different data speeds will mean for you on our Custom plans, Unlimited bundles, and Unlimited All plans. Keep in mind that speeds are “up to” and that the max speeds you get will depend on what device you have, where you are, and...
Remember a time before smartphones ruled our lives? When a phone’s purpose was for calls and texts, the battery lasted for days, and Snake was the height of mobile entertainment. LOL neither do I. But maybe there’s some merit to a less capable phone. ...
The total RNA, which was extracted using TRIZOL (Invitrogen, Carlsbad, CA, USA), was used for cDNA synthesis with MMLV (Moloney murine leukemia virus) reverse transcriptase (Promega, Madison, WI, USA). cDNA was subjected to PCR with primers for PC4 (forward, 5ʹ-GAGCCCTGTCATCTTCTA-3...
Growth factors are proteins that regulate cell division, cell survival, and inflammation, among other cellular functions. Non-limiting examples of growth factors include Adrenomedullin (AM), Angiopoietin (Ang), Autocrine motility factor, Bone morphogenetic proteins (BMPs), Ciliary neurotrophic factor (CN...
Total RNA from the cell lines were extracted with TRIzol reagent (Invitrogen, Carlsbad, CA) and treated with RNase-free DNase (Promega, Madison, WI, USA). Quantitative real-time PCR was performed using the SYBR® Green PCR Master Mix (Applied Biosystems, Foster City, CA, USA). The primer...
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Dual-Glo® Luciferase Assay System (Promega, Madison, WI, USA) was carried out to distinguish luciferase levels after 36 hours of co-transfection. The test was assessed in triplicate. 3. Results 3.1. Characterization of Gonadal mRNAs and miRNAs The samples for mRNA-seq were sequenced from ...
[0151] Total RNA was extracted by Tri-Reagent (Molecular Research Center, Cincinnati, Ohio). For Northern blotting, poly A+mRNA was obtained using oligo dT magnetic columns (Promega, Madison, Wis.). 5-30 &mgr;g mRNA was Northern blotted and probed using standard techniques with probes for ...
18威斯康星大学麦迪逊分校微生物学博士培训项目 University of Wisconsin(Madison) - Microbiology Doctoral Training Program 19加州大学戴维斯分校生物科学学院 University of California(Davis) - College of Biological Sciences 19加州大学洛杉矶分校生态学和进化生物学系 University of California(Los Angeles) - Department...