"Since we have switched from old-school overnight wet transfers to the iBlot 3, we have had much better transfers, less incidences of bubbles, and it has saved us so much time. By transitioning to this method, we have shifted to incubating the nitrocellulose membrane in primary antibody ov...
Blot stained with 0.1% Ponceau S in 5% acetic acid for 5 minutes according to the protocol (Panel B). Electrotransfer systems Several electrotransfer strategies exist. The most common methods are wet, semi-dry and dry, each of...
• If the system has been contaminated by a precipitate, you may need to flush the system with a strong acid or base. 3.2 Initial Screens Power-Up Tests Each time the AD20 power is turned on, the detector runs a series of internal diagnostic and wavelength calibration procedures. During ...
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