METHODS AND RESULTS:Urinary catecholamines were extracted using a cis-diol-specific affinity gel. Biotinylation was performed using amine-reactive NHS ester chemistry, followed by an enzymatic O-methylation.The extract containing the modified catecholamines was incubated with spectrally distinguishable magne...
Results were presented both corrected and uncorrected for creatinine as Total DON ng/mL urine and as g total DON ng/mg creatinine in urine. The latter allow a correction for different degrees of dilution of the urine between individuals. 5.2.6. Dietary Analysis The research dietitians converted ...
Nanobody (Nb), a new type of biorecognition element generally from Camelidae, has the characteristics of small molecular weight, high stability, great solubility and high expression level in E. coli. In this study, with 19-nortestosterone (19-NT), an ana
Following this thermal exposure, colorimetric detection tests were performed using spiked artificial urine, as explained in Section 2.4. UV-vis spectroscopy was used to measure the absorbance at 652 nm over 2, 4, 6, and 24 h, and the results were compared to those of the freshly prepared ...
Results: The addition of Ca(NO3)2 10−4 M promoted the specific adsorption to the metal surface of the anionic purine metabolites such as uric acid, xanthine and hypoxanthine. Moreover, the SERS spectra of urine were acquired without any filtering or processing step for removing protein ...
As the results, the proposed nanobody-based ic-ELISA for 19-NT was demonstrated to be a reliable technology for real sample analysis. FiguFreig1u0r.eS1a0m. pSlaemmpaletrmixaetxritxraecxtstroafctisc-oEfLiIcS-EAL. ISA. TablTea3b.lRee3c.oRveecroyvAernyalAynsiaslyosfi1s9o-fN1T9-iNn T...