UPLC法同时测定阿托伐他汀钙中15个杂质含量 邱荣荣，徐卓兰，杨志明 (常州制药厂有限公司，江苏常州213000)摘要：目的建立超高效液相色谱法(UPLC)同时测定阿托伐他汀钙原料药中15个杂质含量的方法。方法采用岛津Shim-packVeloxPFPP(21mmxioo晌，1.8ym)色谱柱，以3.9g/L醋酸钱缓冲液(pH5.0):乙睛:甲醇:四氢...
Based on UPLC-MS/MS detection platform (UPLC, SHIMADZU Nexera X2, Shimadzu, Kyoto, Japan; MS, Applied Biosystems 4500 Q TRAP, Thermo Scientific), the amides and alkaloids in red huajiao peels were determinated. The UPLC analytical conditions were as follows:1) column, Agilent SB-C18 (1.8 ...
Injections were made using a Shimadzu Nexera XR ultra high-performance liquid chromatograph (UPLC) system (Shimadzu Scientific Instruments, Japan). Mobile phase A was 0.1% formic acid in water. Mobile phase B was 0.1% formic acid in 90:10 acetonitrile/water (v/v). The total running time ...
多生化样品及天然产物都十分复杂,,Waters UPLC 超高效液相色谱,超高效液相色谱仪 Nexera UHPLC LC-30A,通量最大化: 1、 超高速/超高分离度分析 承受最大压力高达130MPa。 2、 超快速进样 通过重叠进行,使进样时间最短,每次进样仅需10秒。 3、 样品容量最大化 Nexera换架器,可处理多达4600种样品;多路系统...
1 材料 1.1 仪器 Nexera UHPLC System 型超快速 UPLC 仪 ,包括 SPD-M20A 型光电二极管阵列检测器(日本 Shimadzu 公 吸道感染,手足口病等[2-4].2015 年版《中国药典》(一部) 司);KQ-3200E 型超声波清洗器(昆山市超声仪器有限 对小儿柴桂退热颗粒的质量控制仅规定了薄层色谱鉴 别和葛根素含量测定,但其...
The non-volatile metabolites were detected using a UPLC-ESI-MS/MS system (UPLC, SHIMADZU Nexera X2; MS, Applied Biosystems 6500 Q TRAP, shimadzu, Kyoto, Japan) equipped with an Agilent SB-C18 chromatographic column (1.8 µm, 2.1 mm × 100 mm). The LC conditions were as follows: mobil...
The UPLC analysis was accomplished using a Shimadzu Nexera X2 UHPLC system (Columbia, MD, USA). The chromatographic separation was achieved using an Acquity UPLC BEH C18column (50 × 2.1 mm, 1.7 μm) with a gradient mobile phase at flow rate of 0.4 mL/min. The sample injection volume ...
The Nexera Ultra-Performance Liquid Chromatograph was purchased from Shimadzu Corporation (Kyoto, Japan). 4.2. Cell Culture Mouse breast cancer 4T1 cells were provided by the Guangdong Provincial Key Laboratory of New Drug Models. 4T1 cells were cultured in DMEM containing 10% fetal bovine serum,...