UPLC-ESI-qMS/MSRecently, a bacterial second messenger, guanosine 5′-diphosphate 3′-diphosphate (ppGpp), has been detected in chloroplasts. However, because ppGpp concentration in plants is much lower than that in bacteria, detailed analysis of ppGpp in plants has not been performed. A ...
1 实验部分 1.1 仪器与试剂 安捷伦1290高效液相色谱—高分辨串联6430四级杆质谱联用仪(UPLC-QQQMS),配有电喷雾电离 源(ESI)(美国安捷伦公司);超声波清洗仪(洁康超声波设备有限公司);高速离心机(安徽中科中佳科 学仪器有限公司);电子天平(丹佛仪器(北京)有限公司)。 对照品绿原酸(批号15082721)和5-咖啡奎宁酸(...
方法:将肉苁蓉干燥品用含内标的甲醇溶液超声提取后,用ZORBAX Eclipse XDB-C18柱(50 mm×2.1 mm,1.8μm)分离,以A相(0.1%甲酸水溶液)-B相(甲醇)作为流动相进行梯度洗脱,利用电喷雾离子(ESI)源,在UPLC-QQQMS的动态多反应模式(Dynamic-MRM)下检测.结果:检测结果显示,同一种肉苁蓉经过冷冻干燥处理后,其苯乙醇...