This study evaluated their phenolic profiles using ultraperformance liquid chromatography-electrospray ionization-high-resolution mass spectrometry (UPLC-ESI-HRMS/MS), identifying 30 phytochemical compounds, including 17 phenolic acids and 13 flavonoids. Novel compounds such as ellagic acid glucoside and 3-...
以2-羟基癸酸为例,图2A显示了其在ESI-模式下的MS / MS光谱。MS / MS光谱中的主要离子(m / z 141.1290)是由中性损失46.0049 Da(图2C)形成的。相反,如图2B所示,3-羟基癸酸的典型产物离子为C2H3O2 -(m / z 59.0138),对...
. The electrospray ionization (ESI) and multiple-reaction-monitoring (MRM) positive ion mode were selected for detection. Multiple characteristic ion pairs were taken for qualitative analysis of the four SCs, with the standard curve method being accepted for quantitative analysis. The results showed t...
信息学和质谱仪器的进步导致了高分辨率质谱(HRMS)的使用增加,包括正交加速飞行时间(oa-TOF)和四极飞行时间(Q-TOF。传统的Q-oa-TOF-MS平台包括一个四极杆(用于前驱体质量选择、MS/MS和定量)、一个碰撞池和一个飞行时间分辨质量分析仪,该分析仪结合了反射子技术以增加飞行时间路径长度和随后的质量分辨力。对于全谱...
性能指标:m/z范围MS:m/z100~4,000Da分辨率8000ESI/MS灵敏度正离子灵敏度:[1Glu]血纤维蛋白肽B(1569Da)2.8fmolS/N>30:1;负离子灵敏度:140pg/μL棉子糖,S/N>200:1;质量测量精确度<5ppm(m/z>4
2.1.3质谱条件电喷雾离子源(ESI),正、负离子同时扫描,电离电压均为3 500 V,鞘气体积流量35个流量单位,辅助气体积流量10个流量单位,毛细管温度320℃,探头加温器温度300℃,数据采集范围m/z100～1 500,质量分辨率70 000。 2.1.4成分指认结果采用UPLC-Q Exactive Orbitrap-HRMS技术,分别在正、负离子模式下对黄连上...
摘要: 目的:利用UPLC-ESI-HRMS"技术对三草保肝汤中化学成分进行分析.方法:采用ACQUITY UPLC BEH C18色谱柱(2.1 mm×100 mm,1.7 μ.m),以甲醇(A)-0.1%甲酸水溶液(B)为流动相进行梯度洗脱(0～2 min,5%A;2～20 min,5%～12%A;20～35 min,12%～40%A;35～38 min,40%... 查看全部>>关键词:...
目的 建立UPLC-ESI-HRMS方法同时测定藏药小大黄Rheum pumilum及酒炙、炒炭、蒸炙3种炮制小大黄中11种成分。方法 色谱采用KinetexTM C18色谱柱(150 mm×4.6 mm,2.6 μm),流动相为乙腈-0.1%甲酸水溶液,梯度洗脱,体积流量0.3 mL/min,进样量1 μL,柱温32℃。质谱采用ESI离子源,负离子模式进行检测。结果 所测...
ESI离子源参数:正负离子Full MS/ddMS2扫描模式;正离子模式下,鞘气流速40 arb,辅助气流速10 arb,辅助气温度320 ℃,离子传输管温度350 ℃,喷雾电压3.5 kV;负离子模式下,鞘气体积流速40 arb,辅助气流速10 arb,辅助气温度320 ℃,离...
2.5. UPLC-ESI-HRMS/MS analysis LC-MS analyses were performed on an Acquity UPLC system class H (Waters, St. Milford, MA, USA) composed of a PDA detector, sample manager and a quaternary solvent manager as well as an Acquity UPLC BEH C18 column (St. Milford, MA, USA; 130 Å, 1.0...