LC(Liquid Chromatography):1903年俄国植物化学家茨维特(Tswett)首次提出“色谱法”(Chromotography)和“色谱图”(Chromatogram)的概念。然而受制造技术和理论发展缓慢的影响,之后液相色谱一度沉寂,没有大步发展。 HPLC(High Performance Liquid Chromatography):上世纪60年代...
赖氨酰肽链内切酶产生了九种不同的肽,其已采用相关标准鉴定,被判定为有效肽(质量精度在预期值的2.5 ppm范围以内,且至少具有3个一级碎片离子)(参见图3)。 图3. LC-MSE chromatograms from thepeptidemapping experiments of EPO A and EPO B digested by Lys-C. The assignment of each chromatographic peak is...
UPLC法测定硫酸羟氯喹原料药有关物质 伍良涌，赵雪梅，李玮玲，董顺玲 （广州市药品检验所，广东广州510160）摘要：目的建立UPLC法有效控制硫酸羟氯喹中的有关物质。方法采用Waters ACQUITY UPLC®BEH C 18 （2.1mm×150mm，1.7μm）色谱柱，以磷酸盐缓冲液（取庚烷磺酸钠0.25g、KH 2PO 4 1.36g，加水900...
After validation, UPLC chromatograms of 21 batches of batches of samples were in good agreement with control fingerprint. 27 batches of samples were clustered into 3 categories, in which S24 was categoryⅠ; S2,S4, S12, S19, S21, S22 were categoryⅡ; other were categoryⅢ. CONCLUSIONS: ...
2.5.2 主成分特征值,方差贡献率 以主成分的特征值 (λ)及方差贡献率作为选取主成分的依据,选取主成分 对应的λ>1 的前几个主成分,共提取到 5 个主成分(见表 Fig 1 UPLC chromatograms of the common peaks of 12 batches of samples 网2.4.2 相似度分析 以 12 批样品共有峰 UPLC 图为标 药房 acy....
黑果腺肋花楸浆果中原花青素B1、原花青素B2、原花青素B4、芦丁和槲皮素的平均质量分数分别为129.78、3 834.99、196.02、134.40、79.10 μg/g。 结论 该方法操作简单、结果准确,具有较好的重复性和稳定性,可用于同时测定黑果腺肋花楸浆果中5种主要有效成分,将为黑果腺肋花楸质量控制提供科学依据。
1 UPLC chromatograms of Lemai Granules sample (a) and mixed reference substances (b) 表 1 线性关系考察结果 Table 1 Results of linear relationship 成分 没食子酸 线性范围 / (μg·mL−1) 回归方程 r 1.80～45.0 Y=11 680 X-5 126 0.999 8 丹参素 1.24～31.0 Y=87 650 X-5 642 0.999 ...
UPLC/MS-MS chromatograms illustrating (a) Standard 4-OH-3OMe-benzylamine (b) Blank (c) Representative Corza sample confirming the presence of 4-OH-3OMe-benzylamine.Terry, G. PowisEmiliano, Gallaga MurrietaRichard, Lesure...
UPLC chromatogram. ES+, of lipid classes extracted from human plasma. 1 E X P E R IM E N TA L Sample Preparation Lipids were extracted from human plasma by adding 30 µL of plasma followed by 180 µL of methanol, and then 360 µL of dichloromethane. The sample was centrifuged ...
图29种化学药对照品定量离子对的MRM色谱图Fig.2MRM chromatograms of quantitative ion pairs of reference substances of nine chemicals 为了保证定量分析的准确度,对质谱扫描条件进行了优化,满足质谱定量对色谱流出峰采集15个点以上的要求。 2.5 专属性考察 ...