Total cellular RNA was isolated using a single step method with TRIzol (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. Real-time PCR was performed in a final volume of 20 μl containing 3 μl of cDNA sample, 20 pmol of each primer and 2X IQ™ SYBR®-G...
There is increasing evidence that many phenotypic properties of a virus and aspects of its interaction with hosts are determined by structural and compositional attributes of its genome. Single stranded RNA virus genomes may be internally base-paired, creating often extensive duplex or higher order str...
These clearcut activities prompted us to design and test novel derivatives on cultured U87-MG and U251 GBM-MG human cells. With the exception of the residues involved in peptide cyclization, residues were Ala-substituted one by one and the single peptides tested for binding affinity for the ...
Structurally, uPA is a single-chain 411 amino acids serine protease. Once secreted, the zymogen inactive form, pro-uPA, is cleaved by plasmin and becomes activated in the extracellular environment [13]. A large body of evidence has indicated that uPA plays an important role in many physiologica...
Structurally, uPA is a single-chain 411 amino acids serine protease. Once secreted, the zymogen inactive form, pro-uPA, is cleaved by plasmin and becomes activated in the extracellular environment [13]. A large body of evidence has indicated that uPA plays an important role in many physiologica...
(B) Endogenous MIC currents have an inward component, a strong outward rectification, and a membrane reversal potential close to 0 mV (averaged trace of nine recordings). (C) Dialyzing of intracellular media with intrapipette solutions containing Mg2+ decreases MIC currents in a dose-dependent ...