which is a core binding sequence from clone 220; 2V is plasmid pRB7-6, containing the MAR ToRB7-6 fragment (SEQ ID NO:20) used as a positive control); 2W is plasmid pGCA887 (containing an insert from yeast ARS1 cloned into the vector pBCKS+(Stratogene)), which serves as a stand...
33.The AAV capsid protein or derivative thereof of claim 30, wherein the rAAV particle is AAV9. 34.A method for altering expression of a transgene operably linked to a promoter and delivered to a cell by a rAAV vector comprising modifying the amino acid sequence of at least one amino ...
Singularities of gradient vector fields in R3: Douglas S. Shafer, Department of Mathematics, University of North Carolina at Charlotte, Charlotte, NC 28223doi:10.1016/0167-2789(83)90055-6ELSEVIERPhysica D Nonlinear Phenomena
In some embodiments, the genetic material is selected from the group consisting of a non-viral gene vector, DNA, RNA, RNAi, and a viral particle. In some embodiments, the particle comprises a biodegradable polymer, wherein the biodegradable polymer is selected from the group consisting of a ...
1. A retroviral vector production system for producing a retroviral vector pseudotyped with influenza HA and M2 that is capable of transducing an airway epithelial target site, comprising a first nucleotide seq
Also, the type of active may vary, for example, it can be characterized as a chemical compound, a small molecule, a ligand, a polypeptide, a nucleic acid (e.g., an antisense nucleic acid construct, a gene therapy vector), or conjugates thereof. In one embodiment, the active is ...
The Geminiviridae family is classified into four genera based on genome structure, insect vector and type of host (Rybicki 1994; Briddon, Bedford et al. 1996). The four genera infect a broad range of plants and cause significant crop losses worldwide (Brown and Bird 1992; Brown 1994; Rybick...
thermophilus following bile exposure by quantification of extracellular beta-galactosidase activity. FIG. 2 illustrates a base vector (pTRK989) used for expressing AQ/AQR rich peptides in St. thermophilus. FIG. 3 illustrates the vectors used for expressing AQ/AQR rich peptides in St. thermophilus...
vector. The 0.17 kb product of the second PCR reaction was digested with HindIII and PstI, and then ligated with a PstI/BamHI fragment of pB1.0 (nt 162 to 632), into HindIII and BamHI sites of pGEM3zf(-) (Promega) to create pG3zNla. This clone contains the first 632 bases of...
Cell lines: The C4-2 cell line was a gift from Dr. Elizabeth M. Wilson (UNC, Chapel Hill, N.C.). C4-2Luc cell line was generated by transfecting C4-2 cells with pTRE2hygro and firefly luciferase (PGL3). PC3 cells were purchased from cell and viral vector core laboratory at Wake ...