University of Mannheim有非常高比例的英语授课课程,因此选择非常多,英语课堂中,仍然可见约一半的德国正规生。学校的主建筑是昔日普法尔茨选帝侯的巴洛克式皇宫,大部分课程都在这裡,教室还保留皇宫裡高窄的门,此外,学校有非常良好的图书馆以及软硬体设施可以充分利用。 2. 暑期语言课 也就是sommerakademie或winterakade...
. Collagenase for hepatocyte isolation was obtained from Boehringer Mannheim (Indiannapolis, Ind.). Vitrogen (Celtrix Labs, Palo Alto, Calif.) was used for the construction of the collagen gels. General reagents were obtained from Sigma Chemical Co. (St. Louis, Mo.). HGF/SF used for these...
The kidney tissue was minced and enzymatically digested using Liberase Blendzyme (Roche, Mannheim, Germany) for 25 minutes at 37 degrees Celsius in a shaking water bath. The supernatant was removed and the cell pellet was passed through a 100 μm cell strainer to obtain a single cell ...
In some of the experiments, plasmid encoding HA-JNK was transfected into BAEC and the exogenous epitope-tagged was immunoprecipitated with a mouse anti-HA mAb (Boehringer Mannheim). The following procedures to assay the activity of the JNK were the same as described above. Plasmids ERK(K52R) ...
. After the chase, the culture medium was collected, centrifuged to remove any insoluble material, and mixed with one tenth volume of 10× lysis buffer (500 mM Tris Cl, pH 7.4 mM MgCl2, 5% NP40, and protease inhibitor cocktail, Complete, Boehringer-Mannheim, GMBH, Germany). To reduce ...
performed at the same settings. The supernatant was removed and the pellet of cells and tissue was resuspended in 50 ml 0.6% Collagenase D (Boehringer Mannheim, Indianapolis, Ind.) in HBSS containing 1 mM CaCl2, gently triturated and then stirred at 37° C. for 15 minutes in an Erlenmeyer...