Briefly, tumor tissues were minced into small pieces with surgical scissors, followed by dissociation into single-cell suspensions with mechanical dissociation and enzymatic degradation of the extracellular matrix with Tumor Dissociation Kit (Miltenyi Biotec, Germany) as per manufacturer’s instructions. ...
Briefly, tumor tissues were minced into small pieces with surgical scissors, followed by dissociation into single-cell suspensions with mechanical dissociation and enzymatic degradation of the extracellular matrix with Tumor Dissociation Kit (Miltenyi Biotec, Germany) as per manufacturer’s instructions. ...