Enzyme Kinetics: Units of kcat? «on:October 07, 2009, 06:19:31 PM » I have my Michaelis-Menten plot of rate (units of mols product/hr/mol enzyme) vs. substrate concentration. From here, I have used a Hanes-Woolf plot to determine Km and Vmax. My Vmax is in units of mols ...
Using GD1a as substrate, the catalytic unit showed a Michaelis-Menten kinetics, and the complex a sigmoid-shaped kinetics, whereas a Michaelis-Menten kinetics was exhibited with MU-NeuAc in both cases. The apparent Vmax and Km values of the catalytic unit for MU-NeuAc and GD1a were 105.1 ...