The most obvious and widely publicized barrier is capital cost, i.e., installing solar and wind farms, expensive catalysts for the biomass to biofuels, etc. Another factor of refrainment is the “siting and maintenance,” which means conventional energy sources such as coal, nuclear are ...
The cell biomass was sonicated in a re-suspension buffer (30 mM Tris-Cl, 0.5 mM EDTA pH 7.5) in the presence of Protease Inhibitor Cocktail, (PIC) (1X) and phenylmethylsulfonyl floride (PMSF) (2 mM) until a clear solution was obtained. Inclusion bodies were pelleted down and washed ...