Using ultra-performance liquid chromatography-tandem mass spectrometry (UHPLC–MS/MS) we found that of the total 2,891 mass features detected by either positive (50.1%) or negative (49.9%) ionization, 447 metabolic features (15.5% of total) were identified and annotated with >70% fragment scor...
Next, tissues 3–9 cm from the root bases were cut into small pieces (2 cm length), frozen in liquid nitrogen, and transported to the laboratory for RNA extraction, transcriptome sequencing and quantitative reverse transcription polymerase chain reaction (qRT-PCR). Soil chemical properties ...
The reaction of o-xylylene complexes of ruthenium with alkynes under oxidative conditionsOxidation of o-xylylene complexes of ruthenium in the presence of dimethyl(acetylenedicarboxylate) produces the dimethylesters of 1,4-dihydronaphthalene-2,3-dicarboxylic acid and naphthalene-2,3-dicarboxylic acid....
morphological, and yield characteristics. Conversely, the biochar addition enhanced all characteristics. Growth-related parameters, such as plant height, stem diameter, leaf area, and dry and wet weight, and leaf gas exchange attributes, such rate of transpiration and photosynthesis, conductivity, as w...
the polymerization. The polymerization was then followed by1H NMR to gain some insights about the polymerization kinetics, which unveiled a first-order kinetics through the course of the reaction (Fig.3b). TheMn,GPCvalues were plotted against the monomer conversion, as shown in Fig.3c, and a ...
This study examined patients aged 18 years and older who were diagnosed with COVID-19 by real‐time fluorescence quantitative polymerase chain reaction assay using pharyngeal/nasopharyngeal swab specimens. Only asymptomatic patients and a small proportion of patients with mild flu-like symptoms were incl...
PrimeScript® RT reagent Kit (TaKaRa), and product was used as templated for RT-qPCR using specific primers (Additional file17). TheCsGAPDHandU6were used to the reference gene. The relative expression levels were calculated by the comparative CT method. All reaction was performed in triplicate...
Total soil bacteria and fungi were quantified using quantitative polymerase chain reaction (qPCR) according to Fierer et al. (2005). To estimate the small-subunit rRNA gene abundances of bacteria and fungi, standard curves were generated using 10-fold serial dilutions of a plasmid containing a fu...
or the mechanisms of bonding and/or charge transfer at interfaces, enabling an understanding of degradation mechanisms at the atomic level and the accurate estimation of some thermodynamic and kinetic properties (e.g., formation energy, binding energy, and energy barriers to diffusion and reaction)....
According to the results of their experiment, although the reaction to not-fully-salient taxes is very heterogeneous among the sample, on average consumers under-react to taxes, i.e. they respond to sales tax as if its size was just 25% of the original tax level. Moreover, as underlined...