1e, f). TDP-43 knockdown led to the inclusion of an annotated frame-shift-inducing exon between exons 10 and 11 in UNC13B, hereafter referred to as the UNC13B frameshift exon (FSE), and increased intron retention between exon 21 and 22 (Fig. 1e, f, Extended Data Fig. 1b). Fig....
FUS allows noninvasive delivery of mechanical energies deep into the tissue and even through the human skull with millimeter-resolution7. FUS by itself
To characterize the expression of each of these isoforms, we fused 4 kb of DNA upstream of the start codon of each isoform in-frame with GFP (Figure 1B). These GFP fusion constructs were used to create transgenic lines, allowing the expression pattern of each isoform to be visualized. The...
For the ELISA experiments, human Unc5D (residues 33–379, Uniprot ID: Q6UXZ4) and human GPC3 (residues 25–563, Uniprot ID: P51654) were cloned into a modified pCMV6-XL4 vector in frame with an N-terminal FLAG and C-terminal human Fc or alkaline phosphatase fragments. ELISA protocol ...
to a stop codon. Both the above mutations should alter the reading frame and truncate the protein. Thirdly, one missense mutation in T19 in exon 8, c.1225G>A which alters Val to Ile . Direct sequencing revealed that many of the mutations identified in the tumours were also present in the...
634 442.9780-01 Lower Fixing Plate CH430 635 442.9776-90 Rubber Compression Mountings For Crusher On Subframe CH430 636 864.0080-00 Rubber Compression Mounting CH430 637 840.0044-00 Screw CH430 638 847.0147-00 Washer CH430 639 442.9780-01 ...
to a stop codon. Both the above mutations should alter the reading frame and truncate the protein. Thirdly, one missense mutation in T19 in exon 8, c.1225G>A which alters Val to Ile . Direct sequencing revealed that many of the mutations identified in the tumours were also present in the...
The cDNA of the respective bait proteins were cloned in-frame with the Gal4-DNA binding domain of pGBKT7. The cDNA of the indicated prey proteins were cloned in-frame with the Gal4 activation domain of pACT2 or pGADT7. The bait and prey plasmids confer tryptophan and leucine prototrophy...