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Equilibrium binding experiments were performed at 25°C using a Biacore T200 instrument (GE Healthcare) using PBS +0.005% (v/v) polysorbate 20 (pH 7.5) or 20mM Tris +200mM NaCl +0.005% (v/v) polysorbate 20 (pH 7.5) as running buffers. Glypican, Unc5 or nanobody constructs were biotin...
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