A fosmid library was constructed to perform gap closure and assembly validation by the Max Plank Institute (MPI) for Developmental Biology using the EpiFOS Fosmid Library Production Kit (Epicentre). End-sequencing of 2880 fosmids was carried out by the MPI for Developmental Biology. Remaining gaps...
DNA was extracted from leaf tissue of a single soil-grown plant using the Qiagen DNeasy Plant Mini Kit and applied to 280-bp and 500-bp paired-end library construction using the NEBNext Ultra DNA Library Prep Kit for Illumina sequencing. Sequencing was performed using the Illumina HiSeq 2500...
[on SciFinder (R)]doi:10.1016/0040-6031(94)02093-4Natascha A. SchillJing-Song LiuUrs von StockarBiotechnology & BioengineeringSchill, N.A., von Stockar, U., 1993. Thermodynamic analysis of Methanobacterium thermoautotrophicum. Thermochim. Acta 251, 71 - 77....
AMRFinderPlus and the reference gene catalog facilitate examination of the genomic links among antimicrobial resistance; stress response; and virulence. Sci. Rep. 2021, 11, 12728. [Google Scholar] [CrossRef] Miral, A.; Jargeat, P.; Mambu, L.; Rouaud, I.; Tranchimand, S.; Tomasi, S....
(ENA), were utilised to identify rDNA repeats. Depending on the sequencing library, the paired-end read length ranged from 150 to 250 bp. The Illumina reads were trimmed to a length of 150 bp, and the high quality of the trimmed reads was ensured by the QC tools of RepeatExplorer2 [42...
LTR (Long Terminal Repeat) elements were detected by LTR_finder [45] and LTR_harvest [46], followed by construction of an LTR library using LTR_retriever [47]. We integrated the two libraries to create a TE library file (TE.lib). To form a comprehensive library, we merged TE.lib with...
The cDNA library was prepared by using 5 μg of starting material for the Illumina mRNA Sequencing Sample preparation procedure (kit RS-930-1001). This involved purification and fragmentation of mRNA, first strand cDNA synthesis, second strand cDNA synthesis, end repair, addition of "A" bases ...
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