were measured via quantitative PCR (qPCR). All qPCR reactions were performed in a total reaction volume of 15 µL containing 1 × Kapa SYBR Fast (PeqLab, Germany), 500 nM unmodified primers, and 1.0 µL of cell-bound ssDNA pool. The amplification reactions were run on a Mastercycl...
Lastly, we evaluated the targeting specificity of enCas12a via Spec-seq/SEAM-seq. As the trade-off for higher non-specific DNA binding affinity, enCas12a displayed reduced binding (Fig.4F) and cleavage specificities (Fig.4H) than both WT (Fig.1D–F; Supplementary Fig.10a, c) and AsCas...
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these effects were related to the presence of individual holes on the target surface, rather than to their spatial distribution or to the reduced mass of the foil. With a greater number of highly energetic electrons crossing the target, enhancement factors of the maximum...
Furthermore, we propose that by arranging three cells that absorb different ranges of the solar spectrum and whose reflection spec- tra compensate for each other, a cascaded configuration can be created that uniquely exploits the spectrum-splitting concept to harvest a broad spectrum of solar light...
The PacBio subreads were error corrected using pacBioToCA16 with the following settings: length 5 500, partitions 5 100, -t 8, pacbio.spec params: ovlHashBlockLength 5 20,000,000, and ovlRefBlockSize 5 5,000,000. Confident corrected subreads that aligned to the maize genome with high...
This NIR extension of the spec- tral responsivity is consistent for all in P3HT photodoped devices and its origin was found to be electric field dependent. A similar voltage dependent NIR response has been seen before in P3HT based photodetectors36 and is consistent with the presence and charge...
Mass Spec. Rev. 2006, 25, 1-53. 5. Agrawal, G.K.; Rakwal, R.; Jwa, N.S.; Agrawal, V.P. Signaling molecules and blast pathogen attack activates rice OsPR1a and OsPR1b genes: A model illustrating components participating during defense/stress response. Plant Physiol. Biochem. 2001, ...