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Lastly, we evaluated the targeting specificity of enCas12a via Spec-seq/SEAM-seq. As the trade-off for higher non-specific DNA binding affinity, enCas12a displayed reduced binding (Fig.4F) and cleavage specificities (Fig.4H) than both WT (Fig.1D–F; Supplementary Fig.10a, c) and AsCas...
were measured via quantitative PCR (qPCR). All qPCR reactions were performed in a total reaction volume of 15 µL containing 1 × Kapa SYBR Fast (PeqLab, Germany), 500 nM unmodified primers, and 1.0 µL of cell-bound ssDNA pool. The amplification reactions were run on a Mastercycl...
:small_orange_diamond: h2spec - is a conformance testing tool for HTTP/2 implementation. :small_orange_diamond: h2t - is a simple tool to help sysadmins to hardening their websites. :small_orange_diamond: htrace.sh - is a simple Swiss Army knife for http/https troubleshooting and profiling...
This NIR extension of the spec- tral responsivity is consistent for all in P3HT photodoped devices and its origin was found to be electric field dependent. A similar voltage dependent NIR response has been seen before in P3HT based photodetectors36 and is consistent with the presence and charge...
Furthermore, we propose that by arranging three cells that absorb different ranges of the solar spectrum and whose reflection spec- tra compensate for each other, a cascaded configuration can be created that uniquely exploits the spectrum-splitting concept to harvest a broad spectrum of solar light...
The PacBio subreads were error corrected using pacBioToCA16 with the following settings: length 5 500, partitions 5 100, -t 8, pacbio.spec params: ovlHashBlockLength 5 20,000,000, and ovlRefBlockSize 5 5,000,000. Confident corrected subreads that aligned to the maize genome with high...
Structured solid targets are widely investigated to increase the energy absorption of high-power laser pulses so as to achieve efficient ion acceleration. Here we report the first experimental study of the maximum energy of proton beams accelerated from