Enzyme-linked Immunosorbent Assay: 1:100-200 Optimal working dilutions must be determined by end user. CONTENTS Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. IHC Images STORAGE Store at 4oC for frequent use. Stored at -20oC to -80oC in ...
AIM: To obtain the active human recombinant uridine diphosphate glucuronosyltransferase 1A3 (UGT1A3) enzyme from Chinese hamster lung (CHL) cells.METHODS: The full-length UGT1A3 gene was amplified by reverse transcription-polymerase chain reaction (RT-PCR) using total RNA from human liver as ...
S8. B In vitro enzyme activities of recombinant AhUGT75A toward various flavonoids with the presence of UDP-glucose. C HPLC analysis of the products from the reaction catalyzed by recombinant AhUGT75A using naringenin, liquiritigenin or hesperetin as substrate. CK was negative control reaction ...
Erythrocyte G6PD activity was quantitated using the enzyme-coupled method described in previous publication (12). MCV was measured by using Sysmex XE 2100 (Sysmex Corp., Tokyo, Japan). Subjects with MCV ≤80 fL were assessed for thalassemia by using a polymerase chain reaction method described i...
The wild-type (normal) (TA) 6/6), low (TA) 7/7, 7/8, 8/8), intermediate (TA) 5/7,5/8, 6/7, 6/8), and high (TA) 5/5, 5/6), enzyme activity genotypes were found in 24.8, 24.8, 41.5%, & 8.9% patients and 20.3, 15.6, 61%, & 3.1% controls respectively. The ...
Direct evidence that sporadic colorectal cancers (CRCs) can arise from an interaction between genes and environment has come from a study of gene variation affecting the power of an enzyme to detoxify known carcinogens. Identification of genetic polymorphisms of the human UDP-glucuronosyltransferase (...
with the former enzyme recognizing a broader range of substrates than the latter. Finally, sequence analysis of populations ofUGT76G1cDNAs revealed that an array of splice variants exists within at least two Stevia cultigens, providing yet another likely form ofUGT76G1regulation. Collectively, the...
UDP-glucuronosyltransferases (UGTs) are membrane proteins present in the endoplasmic reticulum. Human UGTs consist of a phase II metabolic enzyme family of 19 functional enzymes that compose four subfamilies: UGT1 (UGT1A), UGT2 (UGT2A and UGT2B), UGT3, and UGT8 [1,2]. The UGT1A famil...
Google Scholar Albin N, Massaad L, Toussaint C, Mathieu MC, Morizet J, Parise O, Gouyette A, Chabot GG: Main drug-metabolizing enzyme systems in human breast tumors and peritumoral tissues. Cancer Res. 1993, 53: 3541-3546. CAS PubMed Google Scholar Download references ...
(exons 2–5 and 5b) that are shared by all full-length UGT1A transcripts as illustrated inFigure 3(Gonget al.2001; Ritteret al.1992b). As a result, the UGT1Aenzymespossess unique N-terminal ends that providefunctional diversity, while the 245 C-terminalamino acidsof eachenzyme(encoded ...