To investigate the capacity of Drosophila extracts to glucosylate exogenous substrates we have developed a fast and sensitive method for the detection of UDP-glucosyltransferase activity using 4-nitrophenol, 1-naphthol, or 2-naphthol as substrates. High-performance liquid chromatography was used to separ...
UDP-glucose: Flavonol 7-O-glucosyltransferase activity in flower extracts of Chrysanthemum segetum. Z. Naturforsch. C 1997, 52, 153-158. [PubMed]Stich K, Halbwirth H, Wurst F, Forkmann G (1997) UDP-glucose: flavonol 7- O -glucosyltransferase activity in flower extracts of Chrysanthemum ...
GSA1 encodes a UDP-glucosyltransferase, which exhibits glucosyltransferase activity toward flavonoids and monolignols. GSA1 regulates grain size by modulating cell proliferation and expansion, which are regulated by flavonoid-mediated auxin levels and related gene expression. GSA1 is required for the ...
The activity of sucrose phosphate synthetase of different plant tissues is too low to account for the rate of sucrose synthesis occurring in vivo (13). Therefore it could be inferred that the activity of this enzyme might be enhanced by some metabolite. A control at the site of sucrose ...
with theOsActingene being used as an internal control.gHistochemical staining for β-glucuronidase (GUS) activity in germinating seeds. Scale bars represent 10 mm. Inb,d,esignificant differences were determined by two-tailed Student’sttests (*P < 0.05, ***P < 0.001). Source data...
In addition to significant lignin deposits, UGT71C4-KO ovules exhibited greater activity of genes and enzymes related to lignin synthesis; conversely, such activity was significantly suppressed in seeds of UGT71C4-OE. UGT71C4 exhibits specialized glucosyltransferase activity Our findings demonstrated that...
4.3. Glycosyltransferase Assays Glucosyltransferase activity was determined with the UDP-Glo assay from Promega (Fitchburg, WI, USA). All assays were performed at 25 ◦C in 100 mM potassium phosphate buffer at pH 7 and contained 1 mM UDP-glucose and 25 µM of the aglycone. The amount of...
The activity of cerebroside synthase (UDP-glucose: ceramide glucosyltransferase) was found in the microsomal fraction of radish hypocotyls, and was studied. One % of the radioactivity due to UDP[3H]glucose added to the membrane fraction was incorporated into cerebroside within 60 min. Optimum pH ...
This indicates that UGGT activity was present in the ER fraction (Fig. 1a). The activity was abolished when the ER-enriched fraction was inactivated by boiling. We used thyroglobulin as an acceptor to confirm that glucose was transferred from UDP-Glc to high mannose glycoproteins; thyroglobulin ...
Variation of UDP-glucosyltransferase activity, during Drosophila melanogaster development, was analyzed. The endogenous metabolite xanthurenic acid and the... C Rausell,J Llorca,MD Real - 《Archives of Insect Biochemistry & Physiology》 被引量: 27发表: 2010年 Cloning and expression analysis of two...