UDP-Glo Glycosyltransferase Assay + UDP-Glucuronic Acid (UDP-GA) 中文名: 中文别名: CBNumber: CB05761447 分子式: 分子量: 0 MOL File: Mol file 化学性质安全信息用途供应商3 UDP-Glo Glycosyltransferase Assay + UDP-Glucuronic Acid (UDP-GA)化学性质 ...
The reaction mixtures were incubated at 30 °C for 30 min, and were stopped by adding the same volume of UDP-Glo™ assay reagent [47]. To determine the kinetic parameters for naringenin and resveratrol, eight different substrate concentrations ranging from 8 to 500 μM were applied, Vmax ...
Released UDP was detected with the UDP-Glo Assay (Promega). The values shown are the activities relative to deoxynivalenol (DON, 100%). The results are the means of quadruplicate determination ± standard deviations. Substrate Relative Activity (%) OsUGT79 HvUGT13248 Bradi5g03300 Deoxynivalenol ...
中文名称: UDP-Glo Glycosyltransferase Assay + UDP-Glucuronic Acid (UDP-GA) 中文同义词: 英文名称: UDP-Glo Glycosyltransferase Assay + UDP-Glucuronic Acid (UDP-GA) 英文同义词: UDP-Glo Glycosyltransferase Assay + UDP-Glucuronic Acid (UDP-GA) CAS号: 分子式: 分子量: 0 EINECS号: Mol文件:...
Here, we describe a generalized method to detect total UGT activity (intrinsic or induced) via the UGT-Glo assay which utilizes a general UGT substrate with luminescence as the readout eliminating the need for multiple HPLC detectors to detect total UGT activity in a given sample. The method ...
Promoter vectors (pGL4.26, pGL4.50 and pGL4.74) and a Dual-Glo luciferase assay system were purchased from Promega (Madison, WI). All other chemicals and solvents were of analytical grade or the highest grade commercially available. Animals and treatments Tg(UGT1A1*28)Ugt1−/− (hUGT1) ...
A continuous fluorescent enzyme assay for early steps of lipid A biosynthesis Acyl carrier proteinThioGloUDP-N-acetylglucosamine acyltransferase (LpxA) and UDP-3-O-(R-3-hydroxyacyl)-glucosamine acyltransferase (LpxD) catalyze the ... RJ Jenkins,GD Dotson - 《Analytical Biochemistry》 被引量: 14...
The media was replenished every other day for 10 days and cell viability determined using the Cell Titer Glo assay (Promega). CHIR99021 was purchased from Stemcell Technologies. 4.12. Statistics All statistical analyses were performed in R v3.1.0. Fisher's exact test and multiple t-test (...
Poppenberger B, Berthiller F, Lucyshyn D, Sieberer T, Schuhmacher R, Krska R, Kuchler K, Glossl J, Luschnig C, Adam G. Detoxification of the Fusarium mycotoxin deoxynivalenol by a UDP-glucosyltransferase from Arabidopsis thaliana. J Biol Chem. 2003;278(48):47905–14. Article CAS PubMed...
Glycosyltransferases (GTs) are enzymes that catalyze the formation of glycosidic bonds and hundreds of GTs have been identified so far in humans. Glycosyltransferase 8 domain-containing protein 1 (GLT8D1) has been associated with central nervous system d