Briefly, the scale of image was set using Set Scale tool on ImageJ. Followed by measuring the length of ER, PM, or perimeter of mitochondria using freehand line tool. The percent of contact length was determined by taking the ratio of the length of ER, or PM (within contact distances ...
The diameter of LDs was measured using Fiji ImageJ-win32 by drawing the diameter using the ‘line’ tool and measuring it using the ‘measure’ function of the software69. Chloroplast isolation and protein topology analysis Chloroplasts were isolated from plants grown in vitro for 8–10 days af...
u-center is u-blox’ powerful GNSS evaluation and visualization tool which can be downloaded free-of-charge from our website (.u-blox). This user guide provides a description of the features of this software. It allows end users to assess and test u-blox GNSS positioning chips and modules...
(2007). RNA was extracted from embryos by using TRIZol (Invitrogen) and used to generate cDNA (ThermoFisher Scientific, RevertAid First Strand cDNA Synthesis Kit). The PCR was performed with the RedTaq Mastermix (2X, Genaxxon). Amplicons were Phenol extracted and resuspended in 20 μl DEPC ...
UBXD1 mutants (D11A/I12A and R62A/L63A) were produced with the Q5 site-directed mutagenesis kit (New England Biolabs, Frankfurt am Main, Germany) according to the user's manual. Primers used were as follows: UBXD1-ND11A/I12A: fw, 5′-CAGGAGTTCAAAGCGGCCGCGAAGTTCAAGAGCGCC; rv,...
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A specific inhibitor of the protein would be a highly relevant tool to investigate its diverse functional roles and to evaluate its potential as a therapeutic target in human disease. We demonstrate that both overexpression of ASPL and the addition of a cell-permeable truncated ASPL fragment ...