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pET-NTF2 was a kind gift of Dirk Görlich (Max-Planck-Institute for Biophysical Chemistry, Göttingen) and RanQ69LΔC (aa 1–180) from Thomas Monecke and Dirk Görlich51. RanBP2-3/4 mFG variants and the Crm1-Y105A/W880A mutant42 were obtained by (multi)site-directed ...
Plasmids for the bacterial coexpression of FLAG-BRCA1(1–304) and BARD1(26–327) were based on pET28N and pCOT7N expression systems (gift of Michael Wittekind, Bristol-Myers Squibb). For additional details, see the Supplemental Data. Ubiquitination Activity Assays One-hundred microliter ...
Renilla TS was a gift from Nasreldin M. Ibrahim, Otto Fröhlich, and S. Russ Price (Emory University School of Medicine). GAL/GR-525C was constructed using PCR amplification of pSVLGR with the 5′ primer of 5′-CGGGATCCGTGGAGTCTCACAAGACACTTCGGAAAATCCT-3′ and the 3′ primer of 5...
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