More than 50 G1 cells (cyclin A negative) were analyzed for each experiment. Single, double, and triple asterisks indicate p = 1.9 × 10−5, p = 2.9 × 10−5, and p = 4.3 × 10−5, respectively, calculated by Student's t test. Box plots of γH2AX foci at the indicated ...
Cells with ≥10 RNF8 foci per cell were examined to ensure only RNF8-positive cells were analyzed, to avoid inclusion of spurious non-IR induced foci, and increased diffuse background stain in later time points. The experiment was done in triplicate, and data were pooled with at least 120 ...
To assess clone formation ability, cells were seeded in six-well plates, cultured for 24 h, and then exposed to arctigenin or DMSO for 10–12 days. At the end of the experiment, the cells were washed with PBS five times and then fixed with 10% trichloroacetic acid (4 °C). Following...
The mobile fraction is the proportion of Ubc9 in the bleached spine that exchanged with unbleached Ubc9 from the dendritic shaft over the time course of the experiment. In absence of stimulation, there was only a small fraction of immobile Ubc9 in spines with B85% recovery of the initial ...
Data in b–d are the mean of biological replicates from a representative experiment, and error bars indicate s.e.m. Statistical significance in b,d was determined by a two-tailed, unpaired Student's t-test. The experiments were repeated three times. the effect of miR-205 on HR repair, ...
Data in b–d are the mean of biological replicates from a representative experiment, and error bars indicate s.e.m. Statistical significance in b,d was determined by a two-tailed, unpaired Student's t-test. The experiments were repeated three times. the effect of miR-205 on HR repair, ...
The direct interaction between IJ-5 and UbcH5c was assayed by pull-down experiment with IJ-5-coupled beads as described in the Supplemental Experimental Procedures. Determination of IJ-5 Binding Site on UbcH5c The binding site of IJ-5 on UbcH5c was determined using LC-MS/MS analysis perfor...
The data represent two independent experiments, and triplicate transfections were performed in each experiment. (D) Immunoblot detection of Ub conjugates (Ubn), free Ub (Ub1), and GFP in Ubc+/− MEFs treated with 10 μM NaAsO2 or 40 μM tBHQ for up to 10 h. Ubc expression was ...
Use of the hydrolysis-deficient RanQ69L mutant in these experiment should trap intermediate interactions during export complex disassembly, that is, allow formation of a stable Crm1/RanQ69L/RanBD intermediate6. Consistent with this idea were findings with soluble GST-RanBP1 (Fig. 6a): when inc...
In a co-transfection experiment in 293 cells, transfection of neither SAG nor CUL5 alone had any significant effect on the levels of ectopically expressed β- TrCP1. Interestingly, when transfected in combination, SAG-CUL5, but not RBX1-CUL1, significantly reduced the levels of ectopically ...