Yuan R, Hahn Y, Stempel M, Sidibe D, Laxton O, Chen J,et al. Proteasomal inhibition preferentially stimulates lysosome activity relative to autophagic flux in primary astrocytes. Autophagy. 2022;:1-27pubmedpublisher Tao P, Wang S, Ozen S, Lee P, Zhang J, Wang J,et al. Deubiquitination...
To further confirm the interaction between N TIR domain andNbUBR7, we performed GST pull-down assays using recombinant proteins purified fromE. coli. Our results showed that GST-fusedNbUBR7 specifically pulled-down the GFP-fused TIR domain of N, but not the GFP-fused C-terminal region of th...
Functional complementation experiments showed that retroviral expression of the GFP tagged constructs rescued all biochemical defects in cblC and cblD fibroblasts except propionate incorporation in cblD-MMA cells, suggesting that the endogenous mutant protein interferes with the function of the transduced ...
Clearance of damaged mitochondria through mitophagy is critical for maintaining mitochondrial fidelity and the prevention of neurodegeneration. Here, we report on the UBX domain-containing, p97/VCP cofactor UBXD1/UBXN6/UBXDC2 and its role in mitophagy. R
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Using GFP-fusion proteins and life cell imaging, we demonstrate a cytosolic localization for PfAK1, PfAKLP1, and PfAKLP2, whereas PfGAK is located in the mitochondrion. PfAK2 is located at the parasitophorous vacuole membrane, and this localization is driven by N-myristoylation. Structured ...
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c, Purification of 6×His tagged GFP and N-terminal IDR-GFP fusion proteins for in vitro droplet formation assay. d, Visualization of turbidity of indicated protein solutions (20 µM) in droplet formation buffer in the absence (–) or presence (+) of 8% PEG-8000. e, Representative ...
目前常用的报告基因有氯霉素乙酰转移酶基因(cat)、荧光素酶基因(luc)、β-葡萄糖苷酸酶基因(gus)、分泌型碱性磷酸酶基因(seap)、绿色荧光蛋白基因(gfp)等。查看详细∨ 细胞培养基本条件是什么?细胞培养基本条件: 1、合适的细胞培养基合适的小鼠源细胞培养基是体外细胞生长增殖的重要的条件之一,培养基不仅提供细胞...