Clearance of damaged mitochondria through mitophagy is critical for maintaining mitochondrial fidelity and the prevention of neurodegeneration. Here, we report on the UBX domain-containing, p97/VCP cofactor UBXD1/UBXN6/UBXDC2 and its role in mitophagy. R
In addition to classical K48- and K63-linked polyubiquitination and monoubiquitination numerous other ubiquitin chain types are formed. This is possible as all seven lysines in ubiquitin and the N-terminal amine are accessible for linkage and chain formation. Furthermore, ...
Together, they act downstream of K63-linked ubiquitination and p62 recruitment, and selectively remove K48-linked ubiquitin conjugates from a subpopulation of damaged lysosomes to promote autophagosome formation. Lysosomal clearance is also compromised in MEFs harboring a p97 mutation that causes ...
a, The bacterial HECT-like E3 ligase NleL was used with UBE2L3 and ubiquitin(K48R) to produce Lys6-linked chains39. b, The E2 enzyme UBE2S lacking the C-terminal Lys-rich sequence (UBE2S(ΔC)) assembles predominantly Lys11 linkages38. c, The HECT E3 ligase AREL1 with UBE2L3 ...
In addition to classical K48- and K63-linked polyubiquitination and monoubiquitination numerous other ubiquitin chain types are formed. This is possible as all seven lysines in ubiquitin and the N-terminal amine are accessible for linkage and chain formation. Furthermore, homotypic, heterotypic and ...
Adding further combinatorial power to this system is the potential for assembly of polyUb chains with different functions through linkages at any of the seven lysines (K6, K11, K27, K29, K33, K48, and K63) present in Ub [4], as well as the N-terminus of the protein [5]. ...
Here authors show a regulatory cascade of T cell activation, in which DUSP22 negatively regulates UBR2, which is an activator of the kinase Lck via K63 ubiquitination.doi:10.1038/s41467-024-44843-wShih, Ying-ChunChen, Hsueh-FenWu, Chia-Ying...
with the use of a K48R and K63R Ub mutant (for distal Ub) and with the variation of using a Ub with the last two Gly residues deleted (Ub∆GG, for the proximal Ub). K11-linked Ub chains were produced similarly (K11R Ub for distal Ub and Ub∆GG for proximal Ub), with the...