MKP-1 does not function as a general inhibitor of activated transcription as conditional expression of MKP-1 was found to have no effect on PMA-induced activation of a 4X-NFκB-Luc reporter (Fig. 5 E). Furthermore, CdSO4 pretreatment of U937 cells stably expressing the hMTIIa vector alone...
U937 cells grown in a 35 mm dish were transfected for 24 h with 1 μg of each promoter-reporter construct and 1 μg of pSVβGal vector using transfection reagent FuGene 6, according to the instructions given by the manufacturer (Roche Molecular Biochemicals, Indianapolis, IN). After the tra...