Nonviral, host-derived proteins on lentiviral vector surfaces can have a profound effect on the vector's biology as they can both promote infection and provide resistance to complement inactivation. We have exploited this to engineer a specific posttranslational modification of a "nonenvelope," vi...
The most common form of distributional change occurs among migratory animals, which may be plentiful in the summer months and virtually absent in the winter. The forces governing the dispersal of organisms are either vectorial (directed motion), that is, caused by wind, water, or some other ...
“Intestinal parasites and vector-borne pathogens in stray and free-roaming cats living in continental and insular Greece.” PLoS neglected tropical diseases 11.1 (2017): e0005335. Sharif, Mehdi, et al. “Prevalence of intestinal parasites among food handlers of Sari, Northern Iran.” Revista do...
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The fluorescently labeled preparations were mounted in Vectashield (Vector Labs) and viewed on a BioRad MRC1024 or Radiance 2000 confocal microscope. Two-dimensional projections were generated by stacking appropriate sections for each channel using Confocal Assistant software (distributed by Bio-Rad). ...
(clyA) to heterologous antigen expressed in live Salmonella vector demonstrated efficient translocation into periplasmic room and extracellular method. Gathering proof indicates that clyA-mediated antigen distribution enhanced growth fitness and improved immunogenicity of live vector vaccine, however the facets ...
The internal organization of cells depends on mechanisms to ensure that transport carriers, such as vesicles, fuse only with the correct destination organelle. Several types of proteins have been proposed to confer specificity to this process, and we hav
For visualization, cells were incubated with Alexa Fluor 488 Goat Anti-Rabbit antibody (Thermo Fisher Scientific, Waltham, MA) and 4’, 6’-diamidino-2-phenylindole (DAPI, Vector Laboratories, Burlingame, CA) to identify nuclei. Images were acquired on a Leica microscope fitted with ×40 ...
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In this way, the infected vector organism would pass the protozoa from its bloodstream into the host's blood. Protozoa are classified based on how they move in an environment; some are non-motile, while others have modes of transportation such as cilia, flagella, and amoeboid movement. An ...