Improved separation and quantitative determination of hydrocarbon types in gas oil by normal phase high-performance TLC with UV and fluorescence scanning densitometry. Matt M,Galvez E M,Cebolla V.L,Membrado L,Bacaud R,Pessayre S. Journal of Separation Science . 2003...
All cell types were cultivated on treated plastic flasks and multidish plates (Nunc). For microscopy experiments, cells were seeded in glass-bottom dishes (Ibidi) precoated with 0.1% porcine gelatin (Sigma-Aldrich). FA treatment PA, oleic acid, and EPA were dissolved/diluted in sterile DMSO ...
For the MTT assays, 5 × 104 cells were plated onto 24-well plates. At different times, MTT was added at a final concentration of 5 mg/ml. After incubation for 3 h (37 °C, 5% CO2), the blue formazan derivative was solubilized in dimethyl sulfoxide and the absorbance was measured at...
After Rhodamine 6G solution was sprayed onto the TLC plates, each spot was located under UV light and scraped off. Each of the compound isolated by TLC was incubated with 5% methanolic H2S04 for 3 hr at 80掳C and the resulting fatty acid methyl esters and co-hydroxy fatty acid methyl ...
Detection of saturates and aromatics was performed by fluorescence scanning using 365 nm as the excitation wavelength, Alternative detection of aromatics can be performed on either silica gel or berberine-impregnated plates by using ultraviolet (UV) densitometry at 250 nm. On the other hand, polar ...
4.2. MTT Cell Growth Assay 7F2 osteoblasts were seeded at a density of 104 cells/well in 96-well plates to determine cell viability. Culture media were replaced with the media containing test samples (lipids or liposomal formulations) at various concentrations. After 1 day of incubation at 37...