"This platform allows us to isolate specific cell subsets in asample, so we can perform RNA sequencing and address biological questions in different cell types," explained first author Miguel Reyes, a graduate student researcher at the Broad Institute and PhD candidate at MIT. "We've condensed ...
We measure dual single nucleus RNA expression and chromatin accessibility, DNA methylation, and H3K27ac, H3K4me1, H3K4me3, and H3K27me3 histone modifications to decipher the chromatin landscape and gene regulation of the kidney in reference and adaptive injury states. We establish a spatially-...
3f). Whether this observation could be explained by either more frequent or prolonged seasonal infections in patients with SLE remains to be elucidated. Overall, the evaluation of serological responses to mRNA vaccination revealed a defective primary response in SLE that requires vaccine boosters for ...
Single-cell RNA sequencing studies have suggested that total mRNA content correlates with tumor phenotypes. Technical and analytical challenges, however, have so far impeded at-scale pan-cancer examination of total mRNA content. Here we present a method
Rifamycin action on RNA polymerase in antibiotic tolerant Mycobacterium tuberculosis results in differentially detectable populations. Proc. Natl Acad. Sci. USA 114, E4832–E4840 (2017). Article CAS PubMed PubMed Central Google Scholar Vijay, S. et al. Influence of stress and antibiotic ...
These differing responses were explained by steroid-induced changes in RNA content, protein synthesis and protein breakdown.关键词: Animals Rats Muscles Muscle, Smooth Myocardium Dexamethasone RNA Muscle Proteins Muscle Development Kinetics DOI: 10.1042/bj2080147 被引量: 100 ...
Single cell technologies are rapidly generating large amounts of data that enables us to understand biological systems at single-cell resolution. However, joint analysis of datasets generated by independent labs remains challenging due to a lack of consi
Acute perturbation strategies in interrogating RNA polymerase II elongation factor function in gene expression. Genes Dev. 35, 273–285 (2021). Article CAS PubMed PubMed Central Google Scholar Chen, F. X., Smith, E. R. & Shilatifard, A. Born to run: control of transcription elongation ...
This is again in line with the observation that for single residue changes, the effect on biochemical parameters can be explained with a simple model, such as fewer H-bonds lead to lower CLIP affinity and thus to lower thermal stability. Interestingly, apparent CLIP on-rates are fastest for ...
A single-cell and spatially resolved atlas of human breast cancers. Nat Genet. 2021 Sep;53(9):1334-1347. Turan T, Kongpachith S, Halliwill K, et al. A Novel Data Integration Method Identifies Inflamed Tumors from Single-Cell RNAseq Data and Differentiates Cell Type Specific Markers of ...